Recombinant CviJI restriction endonuclease

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor

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435193, 435194, 4353201, 536 232, C12N 1570, C12N 1554

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active

054728720

ABSTRACT:
DNA sequences encoding a novel restriction endonuclease (designated R.C.nu.iJI) and variants thereof are disclosed along with method and materials for production of the same by recombinant methods. A bacterial host cell line transformed with DNA encoding R.C.nu.iJI is also disclosed, as well as methods for expressing R.C.nu.iJI in the bacterial host system and subsequent materials and methods for purification of the enzyme.

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