Recombinant combinatorial genetic library for the production of

Details Recombinant combinatorial genetic library for the production of Recombinant combinatorial genetic library for the production of

1995-06-07

1998-01-27

Patterson, Jr., Charles L.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435148, 435156, 4353201, 4352523, 43525233, 536 231, 536 232, 536 237, C12N 120

Patent

active

057121460

ABSTRACT:
Novel polyketides and novel methods of efficiently producing both new and known polyketides, using recombinant technology, are disclosed. In particular, a novel host-vector system is described which is used to produce polyketide synthases which in turn catalyze the production of a variety of polyketides.

REFERENCES:
patent: 4935340 (1990-06-01), Baltz et al.
Strohl et al. "Significance of anthraquinone formation resulting from the cloning of actinorhodin genes in heterologus streptomycetes" Molecular Microbiology 6(2), 147-152, Jan. 1992.
Rohr, J. "Combinatorial Biosynthesis--An approach in the near future?" Angew. Chem. Int. Ed. Engl. 34 (8), 881-885, May 1995.
Gordon et al., "Strategy and tactics in combinatorial organic synthesis. Application to drug discovery," Acc. Chem. Res. (1996) 29(3):144-154.
Hutchinson, "Drug synthesis by genetically engineered microorganisms," Bio/Technology (1994) 12:375-380.
McAlpine et al., "New antibiotics from genetically engineered actinomycetes. I. 2-Norerythromycines, isolation and structural determinations," The Jrnl. of Antibiotics, (1987) 40(8):1115-1122.
Strohl et al., "Expression of polyketide biosynthesis and regulatory genes in heterologous streptomycetes," J. Ind. Microbiol. (1991) 7:163-174.
Tsoi et al, "Combinatorial biosynthesis of unnatural and natural products: the polykitide example," Chem. Biol. Database Caplus on STN, Chemical Abstract No. 123:169385, (1995) 2(6):355-362.
Bartel et al. (1990) "Biosynthesis of anthraquinones by interspecies cloning of actinorhodin biosynthesis genes in streptomycetes: Clarification of actinorhodin gene functions," J. Bacteriol. 172(9):4816-4826.
Beck et al. (1990) "The multifunctional 6-methylsalicylic acid synthase gene of penicillium patulum. Its gene structure relative to that of other polyketide synthases," Eur. J. Biochem 192:487-498.
Bibb et al. (1989) "Analysis of the nucleotide sequence of the streptomyces glaucescens tcml genes provide key information about the enzymology of Polyketide antibiotic biosynthesis," EMBO J. 8(9):2727-2736.
Caballero et al. (1991) "Organisation and functions of the actVA region of the actinorhodin biosynthetic gene cluster of Streptomyces coelicolor," Mol. Gen. Genet. 230:401-412.
Cortes et al. (1990) "An unusually large multifunctional polypeptide in the erythromycin-produciny polyketide synthase of Saccharopolyspora Erythraea,"Nature (1990) 348:176-178.
Davis et al. (1994) "Functional mapping of a polyketide synthase from aspergillus terreus Involved in lovastatin synthesis," Abst. of the Genetics of Industrial Microorganisms Mtg. P288:192.
Donadio et al. (1991) "Modular organization of genes required for complex polyketide biosynthesis," Science 252:675-679.
Donadio et al. (1992) "Biosynthesis of the erythromycin macrolactone and a rational approach for producing hybrid macrolides," Gene 115:97-103.
Fernandez-Moreno et al. (1991) "The act cluster contains regulatory and antibiotic export genes, direct targets for translational control by the bldA tRNA gene of Streptomyces," Cell 66:769-780.
Fernandez-Moreno et al. (1992) "Nucleotide sequence and deduced functions of a set of cotranscribed genes of streptomyces coelicolor A3(2) including the polyketide synthase for the antibiotic actinorhodin," J. Biol. Chem. 267:19278-19290.
Floss (1991) "Genetic engineering of hybrid antibiotics--a progress report," Tetrahydron 47(31):6045-6058.
Fu (1994) "Engineered biosynthesis of novel polyketides: Stereochemical course of two reactions catalyzed by a polyketide synthase," Biochemistry 33(31):9321-9326.
Hallam (1988) "Nucleotide sequence, transcription and deduced function of a gene involved in polyketide antibiotic synthesis in Streptomyces coelicolor," Gene 74:305-320.
Hershberger et al. (1989) "Genetics and molecular biology of industrial microorganisms," Am. Soc. for Microbiol. (Washington, DC) pp. 68-84.
Hopwood et al. (1985) "Product of `Hybrid` antibiotics by genetic engineering," Nature 314 (6012):642-644.
Katz et al. (1993) "Polyketide synthesis: Prospects for hybrid antibiotics," Ann. Review Microbiol. 47:875-912.
Khosla et al. (1993) "Genetic construction and functional analysis of hybrid polyketide synthases containing heterologous acyl carrier proteins," J. Bacteriol. 175:2197-2204.
Khosla et al. (1992) "Targeted gene replacements in a Streptomyces polyketide synthase gene cluster: role for the acyl carrier protein," Molec. Microbiol. 6(21):3237-3249.
MacNeil et al. (1992) "Complex organization of the Streptomyces avermitilis Genes encoding the avermectin polyketide synthase," Gene 115:119-125).
Malpartida et al. (1984) "Molecular cloning of the whole biosynthetic pathway of a streptomyces antibiotic and its expression in a heterologous host," Nature 309:462-464.
Malpartida et al. (1986) "Physical and Genetic characterisation of the gene cluster for the antibiotic actinorhodin in Streptomyces coelicolor A3(2)," Mol. Gen. Genet. 205:66-73.
Malpartida et al. (1987) "Homology between streptomyces genes coding for synthesis of different polyketides used to clone antibiotic biosynthetic genes," Nature 325(6107):818-821.
McDaniel et al. (1993) "Engineered biosynthesis of novel polyketides," Science 262:1546-1550.
Sherman et al. (1992) "Functional replacement of genes for individual polyketide synthase components in Streptomyces coelicolor A3(2) by hetergenous genes from a different polyketide pathway," J. Bacteriol. 174:6184-6190.
Sherman et al. (1989) "Structure and deduced function of the granaticin-producing polyketide synthase gene cluster of streptomyces violaceoruber Tu22," EMBO J. 8:2717-2725.

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