Recombinant cloning vector, host for the vector, and method for

Details Recombinant cloning vector, host for the vector, and method for Recombinant cloning vector, host for the vector, and method for

1982-10-06

1985-07-16

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

435 70, 435 71, 4351723, 435253, 435317, 536 27, 935 28, 935 29, 935 84, C12P 2100, C12P 2102, C12P 2104, C12N 1500, C12N 120, C12N 100, C07H 2104

Patent

active

045296952

ABSTRACT:
A process for producing organic compounds from an organism is disclosed, together with a recombinant cloning vector and host useful therewith. The invention appears to be most suitable for producing organisms whose growth is inhibited by the product which they produce. In one embodiment of the process, a recombinant cloning vector is prepared which has a gene determinant for the organic compound and an antibiotically inducible control region. The gene determinant is subjected to the control of the control region such that production of the organic compound is inhibited prior to induction. After insertion of the recombinant cloning vector into the host organism, the host is grown to a desired density. Thereafter, production is induced by adding an inducing antibiotic to the host.

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