Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1995-08-07
1998-12-01
Degen, Nancy
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 691, 435 914, 4352523, 4353201, 536 231, C12Q 168, C12P 2100, C12N 1563, C07H 2104
Patent
active
058436561
ABSTRACT:
Cloning systems useful for the isolation of recombinant nucleic acid are disclosed in which the recombination of cloning-system nucleic acid and foreign nucleic acid is linked to the expression of a moiety on the surface of a host organism, the moiety being a first member of a binding pair. When recombination occurs between the nucleic acid and the foreign nucleic acid, the moiety is expressed on the surface of the host organism. The isolation of recombinant nucleic acid is then performed by attaching a second member of the binding pair to a solid support and contacting the host organism with the support. When the first member of the binding pair is expressed on the surface of the host organism, the host organism binds to the second member of the binding pair attached to the solid support, thereby selectively isolating those organisms.
REFERENCES:
patent: 5239062 (1993-08-01), Blattler et al.
Jenkinson and Easingwood. Insertional inactivation of the gene encoding a 76-kilodalton cell surface polypeptide in Streptococcus gordonii challis has a pleiotropic effect on cell surface composition and properties. Infection and Immunity. vol. 58(11), Nov. 30, 1990.
Guilfoyle and Smith. A direct selection strategy for shotgun cloning and sequencing in the bacteriophage M13. Nucleic Acids Res.. vol. 22(1):100-107, Jan. 30, 1994.
Bernard et. al.. Positive-selection vectors using the F plasmid ccdB killer gene. Gene. vol. 148(1), Oct. 21, 1994.
Bernard et al. Gene 148: 71-74 (1994) Positive-selection vectors using the F plasmid ccdB killer gene.
Austin et al. Biotechniques 18(3):380-384 (1995) Visual Detection Method for Identifying Recombinant Bacterial Colonies.
Shabarova et al. Nucleic Acids Research 19 (15):4247-4251 (1991) Chemical ligation of DNA: The first non-enzymatic assembly of a biologically active gene.
Herrlein et al. Nucleic Acids Research 22 (23):5076-5078 (1994) Selective chemical autoligation on a double-stranded DNA template.
Peterson et al. Cell 54: 65-72 (1988) Genetic analysis of Monoclonal Antibody and HIV Binding Sites on the Human Lymphocyte Antigen CD4.
Devlin et al. Science 249: 404-406 (1990) Random Peptide Libraries: A source of Specific Protein Binding Molecules.
Houghten et al. Nature 354: 84-86 (1991) Generation and use of synthetic peptide combinatorial libraries for basic research and drug discovery.
Wysocki et al. Proc. Natl. Acad. Sci. USA 75(6):2844-2848 (1978) "Panning" for lymphocytes: A method for cell selection.
Zheng et al BioTechniques 18(5):740-741 (1995) Immunological screening of homologous recombination in genes that encode surface antigens.
Scott et al Science 249:386-390 (1990) Searching for Peptide Ligands with an Epitope Library.
Lutz et al. Proc. Natl. Acad. Sci. USA 84:4379-4383 (1987) Syrinx 2A: AN improved .lambda. phage vector designed for screening DNA libraries by recombination in vivo.
Stengelin et al. EMBO Journal 7(4): 1053-1059 (1988) Isolation of cDNAs for two distinct human Fc receptors by ligand affinity cloning.
Uber et al. BioTechniques 11(5):642-648 (1991) Application of Robotics and Image Processing to Automated Colony Picking and Arraying.
Jones et al. Nucleic Acids Research 20 (17): 4599-4606 (1992) Integration of image analysis and robotics into a fully automated colony picking and plate handling system.
Aruffo et al Proc. Natl. Acad. Sci. USA 84: 8573-8577 (1987) Molecular cloning of a CD28 cDNA by a high-efficiency COS cell expression system.
Huse et al Science 246: 1275-1281 (1989) Generation of a Large Combinatorial Library of the Immunoglobin repertoire in Phage Lambda.
Messing et al Proc. Natl. Acad. Sci. USA 74(9): 3642-3646 (1977) Filamentous coliphage M13 as a cloning vehicle: Insertion of a HindII fragment of the lac regulatory region in M13 replicative form in vitro.
Messing Methods in Enzymology 101: 20-78 (1983) New M13 Vectors for Cloning.
Aruffo et al EMBO Journal 6(11):3313-3316 (1987) Molecular cloning of two CD7 (T-cell leukemia antigen) cDNAs by a COS cell expression system.
Seed et al Proc. Natl. Acad. Sci. USA 84: 3365-3369 (1987) Molecular cloning of the CD2 antigen, the T-cell erythrocyte receptor, by a rapid immunoselection procedure.
Hochuli Genetic Engineering 12: 87-98 (1990) Purification of Recombinant Proteins with Metal Chelate Adsorbent.
Singhvi et al Science 264: 696-698 (1994) Engineering Cell Shape and Function.
Edlind et al. Plasmid expression vector using the lambda late promoter. Plasmid vol. 15:242-244, May 1, 1986.
Meyenberg et al. Promoters of the atp operon coding fro teh membrane-bound ATP synthase of Escherichia coli mapped by Tn10 insertion mutations. Mol. Gen. Genet. vol. 188:240-248, Dec. 2, 1988.
Solenberg et al. Method for selection of transposable DNA and characterization of a new insertion sequence, IS493, from Streptom;yces lividans. J. Bact. vol. 171(9):4807-4813, Sep. 1989.
Shen et al. Dominant negative reinoic acid receptor beta. Mech. Dev. vol. 40:177-189, Apr. 22, 1993.
Chou et al. Mapping of herpes virus-1 neurovirulence to gamma1-34.5, a gene nonessential for growth in culture. Science vol. 250:1262-1266, Nov. 30, 1990.
Ward et al. Vectors for Cu++-inducible production of glutathioine S-transferase-fusioin proteins for single-step purification from yeast. Yeast. vol. 10:441-449, May 18, 1994.
Brandis John
Bridgham John A.
Hoeprich, Jr. Paul D.
Leong John
Bortner Scott R.
Degen Nancy
Grossman Paul D.
Sandals William
The Perkin-Elmer Corporation
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