Recombinant Chlamydia trachomatis pgp3 fusion protein

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

435 691, 435 697, 4241841, 4241851, 4241921, 4242631, 530300, C12P 2106, C12P 2104, A61K 3900, A61K 3800

Patent

active

061107050

ABSTRACT:
A plasmid isolated from Chlamydia trachomatis is described, which comprises 8 genes encoding proteins useful in the formation of vaccines or diagnostic test for determining the bacterium or specific antibodies generated during C. trachomatis infections. In particular, the recombinant fusion protein MS2-pgp3D is described, which comprises polypeptide sequences encoded by pCT and is immunogenic in the course of infections in man. A method for preparing the recombinant fusion protein MS2-pgp3D in E. coli is also described.

REFERENCES:
Klinkert et al Molecular & Biochemical Parasitology 27: 233-240, 1988.
Database Search of Sequence in Plasmid: 23: 149-154 1990.
Palmen et al Plasmid 16: 52-62 1986.
Maniatis et al Molecular Cloning A Laboratory Manual.
Caldwell, H. et al., "Purification and Partial Characterization of the Major Outer Membrane Protein of Chlamydia trachomatis", Infection and Immunity 1981, 31 (3), 1161-1176.
Cevenini, R. et al., "Effects of Penicillin on the Synthesis of Membrane Proteins of Chlamydia trachomatis LGV2 Serotype", FEMS Microbiol. Letters 1988, 56, 41-46.
Davis, L.G., "Basic Methods in Molecular Biology", Elsevier Edit., New York, 1986.
Mandel, M. and Higa, "Calcium-Dependent Bacteriophage DNA Infection", J. Mol. Biol. 1970, 53, 159-162.
Nicosia, A. et al., "Expression and Immunological Properties of the Five Subunits of Pertussis Toxin", Infection and Immunity 1987, 55 (4), 963-967.
Remaut, E. et al., "Improved Plasmid Vectors with a Thermoinducible Expression and Temperature-Regulated Runaway Replication", Gene 1983, 22, 103-113.
Saiki, R. et al., "Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase", Science 1988, 239, 487-491.
Sanger, F. et al., "DNA Sequencing with Chain-Terminating Inhibitors", PNAS USA 1977, 74(12), 5463-5467.
Strebel, K. et al., "Characterization of Fott-and-Mouth Disease Virus Gene Products with Antisera Against Bacterially Synthesized Fusion Proteins", J. of Virology 1986, 57(3), 983-991.
Wang, S. and Grayston, "Immunologic Relationship Between Genital Tric, Lymphogranuloma Venereum, and Related Organisms in a New Microtiter Indirect Immunofluorescence Test", Am. J. of Ophthamology 1970, 70(3), 367-374.
Comanducci, M. et al., "Identification and Characterization of a 28-Kd Protein of C. trachomatis Encoded by the 7.5-Kb Common Plasmid", in "International Symposium on Human Chlamydial Infections", Jun. 24-29, 1990, pp. 121-124.
Comanducci, M. et al., "The Structure of a Plasmid of Chlamydia trachomatis Believed to be Required for Growth Within Mammalian Cells", Mol. Microb. 1988, 2(4), 531-538.
Comanducci, m. et al., "Diversity of the Chlamydia trachomatis Common Plasmid in Biovars with Different Pathogenicity", Plasmid 1990, 23, 149-154.
Morrison, R. et al., "Immunology of Chlamydia trachomatis Infections", in Sexually Transmitted Diseases, Quinn, T., ed., Raven Press, Ltd., New York, 1992, pp. 57-84.
Zhang, Y.-X. et al., "Protective Monoclonal Antibodies to Chlamydia trachomatis Serovar--and Serogroup-Specific Major Outer Membrane Protein Determinants", Infection and Immunity 1989, 57(2), 636-638.
Maniatis et al., "Molecular Cloning A Laboratory Manual", 1982.
Palmer et al., "A Common Plasmid of Chlamydia trachomatis", Plasmid 1986, 16, 52-62.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Recombinant Chlamydia trachomatis pgp3 fusion protein does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Recombinant Chlamydia trachomatis pgp3 fusion protein, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Recombinant Chlamydia trachomatis pgp3 fusion protein will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-1248565

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.