Recombinant chimeric HIV-1 Gag-Env fusion proteins

Details Recombinant chimeric HIV-1 Gag-Env fusion proteins Recombinant chimeric HIV-1 Gag-Env fusion proteins

1995-01-18

1996-11-19

Budens, Robert D.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

4241881, 4241921, 435 71, 4351723, 435974, C07K 100, C07K 1400, C07K 1700, A61K 3921

Patent

active

055764215

ABSTRACT:
Disclosed is the construction, expression, and purification of chimeric human immunodeficiency virus type 1 (HIV-1) Gag-Env fusion proteins. Gag-Env chimeras were generated by fusing the amino terminus (amino acids 512-611) of the Env protein to the carboxyl terminus of the Gag protein (either amino acids 121-406 or 308-406). These proteins were overexpressed in Escherichia coli, purified, and their immunologic properties ascertained. Both chimeric proteins displayed immunoreactivity towards antisera obtained from HIV-1 seropositive patients. These HIV-1 Gag-Env fusion proteins should provide useful antigens for the detection of HIV-1-specific antibodies.

REFERENCES:
patent: 4925784 (1990-05-01), Crowl et al.
Shoeman, et al, 1987, "Comparison of recombinant human immunodeficiency . . " Analytical Biochem. 161:370-379.
Siitari et al. (1990) Journal of Clinical Microbiology 28(9):2022-2029.
Ellinger, et al., Virology (1991), 180(2):811-813.

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