Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification
Patent
1998-08-14
2000-08-22
Brusca, John S.
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
4352523, 43525233, 536 237, 536 241, C12N 1574, C12N 121, C12N 1531, C12N 1552
Patent
active
061070939
ABSTRACT:
Recombinant host cells are obtained that comprise (A) a heterologous, polypeptide-encoding polynucleotide segment, stably integrated into a chromosome, which is under transcriptional control of an endogenous promoter and (B) a mutation that effects increased expression of the heterologous segment, resulting in enhanced production by the host cells of each polypeptide encoded by that segment, relative to production of each polypeptide by the host cells in the absence of the mutation. The increased expression thus achieved is retained in the absence of conditions that select for cells displaying such increased expression. When the integrated segment comprises, for example, ethanol-production genes from an efficient ethanol producer like Zymomonas mobilis, recombinant Escherichia coli and other enteric bacterial cells within the present invention are capable of converting a wide range of biomass-derived sugars efficiently to ethanol.
REFERENCES:
patent: 4321328 (1982-03-01), Hoge
patent: 4493893 (1985-01-01), Mielenz et al.
patent: 4612287 (1986-09-01), Coleman et al.
patent: 4839286 (1989-06-01), Backman
Alterthum, F. and Ingram, L. O.: Efficient Ethanol Production from Glucose, Lactose, and Xylose by Recombinant Escherichia coli. App. Env. Microbiol. (1989) 55:1943-1948.
Al-Zaag, "Molecular Cloning or Cellobiose and Other .beta.-Glucosidase Determinants from Klebsiella Oxytoco," J. Biotechnol. 12:79-86 (1989).
Barbosa et al., Current Microbiol. 28:279-282 (1994).
Beall, D. S., L. O. Ingram (1993) "Genetic Engineering of Soft-rot Bacteria for Ethanol Production from Lignocellulose" J. Ind. Microbiol. 11:151-155.
Biely, Trends Biotechnol. 3:286-290 (1985).
Brau, B. and H. Sahm (1986) "Cloning and Expression of the Structural Gene for Pyruvate Decarboxylase of Zymomonas mobilis in Escherichia coli," Arch. Microbiol. 144:296-301.
Bringer-Meyer, S., K.-L. Schimz, and H. Sahm (1986) "Pyruvate Decarboxylase from Zymomonas mobilis. Isolation and Partial Characterization," Arch. Microbiol. 146:105-110.
Brock et al., Biology of Microorganisms, 4th Edition, Prentice-Hall, Inc., Englewood Cliffs, N.J., 1984, pp. 803-805.
Coleman et al., J. Bacteriol. 169:4302-4307 (1987).
Conway et al., "Expression Vector for Zymomonas mobilis", Appl. Environ. Microbiol., Feb. 1987, vol. 53, No. 2, pp. 235-241.
Conway, T. et al.: Cloning and Expression of Ethanologenic and Glycolytic Genes from Zymomonas mobilis in E. coli. Abstr. Annu. Meet. Am. Soc. Microbiol. 1987, p. 159.
Conway, T., G. W. Sewell, Y. A. Osman and L. O. Ingram (1987) "Cloning and Sequencing of the Alcohol Dehydrogenase II Gene from Zymomonas mobilis," J. Bacteriol. 169:2591-2597.
Conway, T., Y. A. Osman, J. I. Konnan, E. M. Hoffmann, and L. O. Ingram (1987) "Promoter and Nucleotide Sequences of the Zymomonas mobilis Pyruvate Decarboxylase," J. Bacteriol. 169:949-954.
Cornet et al., "Characterization of Two Cel(Cellulose Degradation) Genes of Clostridium Thermocellum Coding for Endoglucanases", Bio/Technology, Sep. 1983, pp. 589-594.
Curry et al., "Expression and Secretion of a Cellulomonas fimi Exoglucanase in Saccharomyces cerevisiae", Appl. Environ. Microbiol., Feb. 1988, vol. 54, No. 2, pp. 476-484.
Danilevich et al., Molecular Biology 28:158-166 (1994).
Esser, K. et al.: Proc. Biochem. 17(3):46-49 (1982).
Fried, V. A., A. Novick (1973) "Organic Solvents as Probes for the Structure and Function of the Bacterial Membrane: Effects of Ethanol on the Wild Type and an Ethanol-Resistant Mutant of Escherichia coli K-12," J. Bacteriol. 114(1):239-248.
Gilkes, N. R. et al.: A Mutant of Escherichia coli that Leaks Cellulase Activity Encoded by Cloned Cellulose Genes from Cellulomonas fimi. Bio/Technol. (1984)2:259-263.
Gold et al., J. Ind. Microbiol. 10:45-54 (1992).
Gong, C.-S. et al. (1981) "Production of Ethanol from D-Xylose by Using D-Xylose Isomerase and Yeasts" Applied and Environmental Microbiology 41(2):430-436.
Grepinet et al., "Nucleotide Sequence and Deletion Analysis of the Xylanase Gene (xynZ) of Clostridium thermocellum", J. Bacteriol., vol. 170, No. 10, Oct. 1988, pp. 4582-4588.
Grepinet et al., (1988) Purification of Clostridium thermocellum Xylanase 2 Expressed in Escherichia coli and Indietificationof the Corresponding Product in the Culture Mediumof C. thermocellum J. Bacteriol. 170:4576-4581.
Hall, B. G. and Faunce, W.: Functional Genes for Cellulose Utilization in Natural Isolates of Escherichia coli. J. Bacteriol. (1987) 169:2713-2717.
Hamada, H. (1986) "Activation of an Enhancerless Gene by Chromosomal Integration" Mol. and Cell Bio. 6:4179-184.
Hashiba et al., Biosci. Biotech. Biochem. 56:190-194 (1992).
Herbert, R. A.; Trends Biotechnol. 10:395-402 (1992).
Ingram, L. O. (1976) "Adaptation of Membrane Lipids to Alcohols," J. Bacteriol. 125(2):670-678.
Ingram, L. O. and T. Conway (1988) "Expression of Different Levels of Ethanologenic Enzymes from Zymomonas mobilis in Recombinant Strains of Escherichia coli," Appl. Environ. Microbiol. 54:397-404.
Ingram, L. O., N. S. Vreeland (1980) "Differential Effects of Ethanol and Hexanol on the Escherichia coli Cell Envelope," J. Bacteriol. 144(2):481-488.
Ingram, L. O., T. M. Buttke (1984) "Effects of Alcohols on Micro-Organisms," in Advances in Microbial Pathology, vol. 25, Academic Press, London, pp. 254-295.
Joliff et al., "Isolation, Crystallization and Properties of a New Cellulase of Clostridium thermocellum Overproduced in Escherichia coli" Bio/Technology, vol. 4, Oct. 1986, pp. 896-890.
Koide, Y. et al. (1986) "Cloning and Sequencing of the Major Intracellular Serine Protease Gene of Bacillus subtilis" Journal of Bacteriology 167(1):110-116.
Kotoujansky, A. et al: Molecular Cloning of Erwinia Chrysanthemi Pectinase and Cellulase Structural Genes, Embo J. (1985)4:781-785.
Lawford et al., Appl. Biochem. Biotechnol. 28/29:221-236 (1991).
Lawford, H.G. et al.: Biotechnol. Lett. 13:191-196 (1991).
Mes-Hartree et al., Can. Bioenergy R. & D. Seminar (Proc.), 5th, 1984, pp. 469-472, Elsevier Appl. Sci, London.
Millet, J. et al.: Cloning of Ten Distinct DNA Fragments of Clostridium thermocellum Coding for Cellulases. Fems Microbiol. Lett. (1985) 29:145-149.
Mistry, F. R., C. L. Cooney (1989) "Production of Ethanol by Clostridium thermosaccharolyticum: II. A Quantitative Model Describing Product Distrubutions" Biotechnology and Bioengineering 34:1305-1320.
Mistry, F.R., C.L. Cooney (1989) "Production of Ethyanol by Clostridium thermosaccharolyticum: I. Effect of Cell Recycle and Environmental Parameters" Biotechnology and bioengineering 34:1295-1304.
Neale et al., Appl. Microbiol. Biotechnol., 29:162-67 (Feb. 1988).
Neale, A. D., R. K. Scopes, R. E. H. Wettenhall and N. J. Hoogenraad (1987) "Nucleotide Sequence of the Pyruvate Decarboxylase Gene from Zymomonas mobilis," Nucleic Acids Res. 15:1753-1761.
O'Hara, M. B., J. H. Hageman (1990) "Energy and Calcium Ion Dependence of Proteolysis during Sporulation of Bacillus subtilis Cells," Journal of Bacteriology 172(8):4161-4170.
Ohta et al., (1991) "Metabolic Engineering of Klebsiella oxytoca M5A1 for Ethanol Production from Xylose and Glucose," Appl. Env. Microbiol. 57:2810-2815.
Panbangred et al., Appl. Microbiol. Biotechnol. 22:259-264 (1985).
Petre et al., "Purification asnd Properties of the Endoglucanase C of Clostridium thermocellum Produced in Escherichia coli", Biochimie, vol. 68, 1986, pp. 687-695.
Raven, P. H. et al.; Biology of Plants, Worth Publishers, Inc., New York, 1981, pp. 251-255.
Sarthy, A. V. et al. (1987) "Expression of the Escherichia coli Xylose Isomerase Gene in Saccharomyces cerevisiae" Applied and Environmental Microbiology 53(9):1996-2000.
Sneath et al., Eds., Bergey's Manual of Systematic Biology, vol. 2, Williams & Wilkins, Baltimore, Md., 1986, pp. xxi-xxiii.
Tailliez et al., "Cellulose Fermentation by an Asporogenous Mutant and an Ethanol-Tolerant Mutant of Clostridium thermolocellum", Appl. Environ. Microbiol., vol. 55, No. 1, Jan. 1989, pp. 203-206.
Tolan, J. S. and R. K. Finn (1987) "Fermentation of D-Xylose and L-Arabinos to Ethanol by Erwinia chrysanthemi," Appl. Environ. Microbiol. 53:2033-2038.
T
Ingram Lonnie O.
Ohta Kazuyoshi
Wood Brent E.
Brusca John S.
Hanley, Esq. Elizabeth A.
Lauro, Esq. Peter C.
University of Florida Research Foundation Inc.
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