Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification
Reexamination Certificate
2007-03-20
2007-03-20
Jones, W. Gary (Department: 1655)
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
C435S252300, C435S252330, C536S023700, C536S024100
Reexamination Certificate
active
09620060
ABSTRACT:
Recombinant host cells are obtained that comprise (A) a heterologous, polypeptide-encoding polynucleotide segment, stably integrated into a chromosome, which is under transcriptional control of an endogenous promoter and (B) a mutation that effects increased expression of the heterologous segment, resulting in enhanced production by the host cells of each polypeptide encoded by that segment, relative to production of each polypeptide by the host cells in the absence of the mutation. The increased expression thus achieved is retained in the absence of conditions that select for cells displaying such increased expression. When the integrated segment comprises, for example, ethanol-production genes from an efficient ethanol producer likeZymomonas mobilis, recombinantEscherichia coliand other enteric bacterial cells within the present invention are capable of converting a wide range of biomass-derived sugars efficiently to ethanol.
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Ingram Lonnie O.
Ohta Kazuyoshi
Wood Brent E.
Chakrabarti Arun Kr.
Edwards Angell Palmer & & Dodge LLP
Jones W. Gary
Lauro, Esq. Peter C.
The University of Florida Research Foundations, Inc.
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