Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1984-11-23
1989-05-16
Martinell, James
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
435 91, 4351721, 4351723, 536 28, 536 29, C07H 2100, C07H 2102, C07H 2104, C12P 1934, C12N 1500
Patent
active
048311243
ABSTRACT:
Compounds useful as complementary DNA (cDNA) include deoxyribonucleotides and at least one ribonucleotide. They may be depicted by the general formula: ##STR1## wherein (dN).sub.a and (dN).sub.c represent series of deoxyribonucleotides and (rN).sub.b represents a series of ribonucleotides; wherein a, b, and c are the number of nucleotides in the series, with the proviso that b is .gtoreq.1, a is .gtoreq.35, and c is .gtoreq.10; wherein the series of deoxyribonucleotides (dN).sub.a includes a series of deoxyribonucleotides which is substantially complementary to the series of deoxyribonucleotides (dN).sub.c and the dashed line represents non-covalent bonding between the complementary deoxyribonucleotide series; and wherein the solid line represents a covalent phosphodiester bond.
These compounds may be prepared from messenger RNA (mRNA) containing the genetic information necessary for cellular production of desired products such as polypeptides. After appropriate modification, they may be combined with DNA from a suitable cloning vehicle such as a plasmid and the resulting combined DNA used to transform bacterial cells. The transformed bacterial cells may then be grown and harvested; and the desired product or products recovered.
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Martinell James
Research Corporation Technologies
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