Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1980-12-10
1982-12-07
Tanenholtz, Alvin E.
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
536 28, 435 68, 435 70, 435 91, 435172, 435317, C07H 1512, C12P 2100, C12P 2102, C12P 1934, C12P 1500, C12N 100
Patent
active
043628672
ABSTRACT:
Compounds useful as complementary DNA (cDNA) include deoxyribonucleotides and at least one ribonucleotide. They may be depicted by the general formula: ##STR1## wherein (dN).sub.a and (dN).sub.c represent series of deoxyribonucleotides and (rN).sub.b represents a series of ribonucleotides; wherein a, b, and c are the number of nucleotides in the series, with the proviso that b is .gtoreq.1, a is .gtoreq.35, and c is .gtoreq.10; wherein the series of deoxyribonucleotides (dN).sub.a includes a series of deoxyribonucleotides which is substantially complementary to the series of deoxyribonucleotides (dN).sub.c and the dashed line represents noncovalent bonding between the complementary deoxyribonucleotide series; and wherein the solid line represents a covalent phosphodiester bond.
These compounds may be prepared from messenger RNA (mRNA) containing the genetic information necessary for cellular production of desired products such as polypeptides. After appropriate modification, they may be combined with DNA from a suitable cloning vehicle such as a plasmid and the resulting combined DNA used to transform bacterial cells. The transformed bacterial cells may then be grown and harvested; and the desired product or products recovered.
REFERENCES:
Salser, in Genetic Engineering, Chakrabarty (ed.), CRC Press, West Palm Beach, Fla., pp. 53-81 (1978).
Kavsan et al., Chem. Abstr. 92, 224 123526h (1980).
Romano et al., Chem. Abstr. 91:170822r (1979).
Hartman et al., Chem. Abstr. 87:147246q (1977).
Waqar et al., Chem. Abstr. 84:100898b (1976).
Ogawa et al., Chem. Abstr. 87, 17513n (1977).
Martinell James
Research Corporation
Tanenholtz Alvin E.
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