Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Patent
1982-11-22
1985-03-05
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
435 91, 435 70, 4351723, 435240, 435241, 435255, 435256, 435317, 435253, 536 27, 536 28, 536 29, 935 2, 935 27, 935 29, 935 60, C12P 1934, C12P 2100, C12P 2102, C12N 1500, C12N 500, C12N 502, C12N 116, C12N 118, C12N 100, C12N 120, C07H 2102, C07H 2104
Patent
active
045031517
ABSTRACT:
Compounds useful as complementary DNA (cDNA) include deoxyribonucleotides and at least one ribonucleotide. They may be depicted by the general formula: ##STR1## wherein (dN).sub.a and (dN).sub.c represent series of deoxyribonucleotides and (rN).sub.b represents a series of ribonucleotides; wherein a, b, and c are the number of nucleotides in the series, with the proviso that b is .gtoreq.1, a is .gtoreq.35, and c is .gtoreq.10; wherein the series of deoxyribonucleotides (dN).sub.a includes a series of deoxyribonucleotides which is substantially complementary to the series of deoxyribonucleotides (dN).sub.c and the dashed line represents noncovalent bonding between the complementary deoxyribonucleotide series; and wherein the solid line represents a covalent phosphodiester bond.
These compounds may be prepared from messenger RNA (mRNA) containing the genetic information necessary for cellular production of desired products such as polypeptides. After appropriate modification, they may be combined with DNA from a suitable cloning vehicle such as a plasmid and the resulting combined DNA used to transform bacterial cells. The transformed bacterial cells may then be grown and harvested; and the desired product or products recovered.
REFERENCES:
Salser: in Genetic Engineering, Chakrabarty (ed.), CRC Press, West Palm Beach, Fla., pp. 53-81, (1978).
Kavsan et al.: Chem. Abstr. 92: 123526h, (1980).
Romano et al.: Chem. Abstr. 91: 170822r, (1979).
Hartman et al.: Chem. Abstr. 87: 147246q, (1977).
Waqar et al.: Chem. Abstr. 84: 100898b, (1976).
Ogawa et al.: Chem. Abstr. 17512n, (1977).
Martinell James
Research Corporation
Wiseman Thomas G.
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