Recombinant Candida rugosa lipases

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

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C435S196000, C435S071100

Reexamination Certificate

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07468429

ABSTRACT:
The present invention features an isolated nucleic acid that includes a mutant DNA encoding aCandida rugosalipase, wherein the mutant DNA is 80% identical to a wild-type DNA encoding theCandida rugosalipase, and includes at least 12 (e.g., 13, 15, 17, or all) universal serine codons corresponding to CTG codons in the wild-type DNA. Each of the universal serine codons, independently, is TCT, TCC, TCA, TCG, AGT, or AGC. TheCandida rugosalipase can beCandida rugosalipase 1, 2, 3, 4, 5, or 8.

REFERENCES:
patent: WO 99/14338 (1999-03-01), None
Longhi et al. Cloning and nucleotide acid sequences of two lipase genes fromCandida cylindracea. Biochimica et Biophysica Acta 1992, 1131, 227-232.
Diczfalusy et al, Isolation of carboxylester lipase (CEL) isoenzymes fromCandida rugosaand identification of the corresponding genes. Arch Biochem Biophys. Dec. 1, 1997;348(1):1-8.
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