Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Reexamination Certificate
2006-05-30
2006-05-30
Nashed, Nashaat T. (Department: 1656)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
C435S254230, C435S252300, C435S254200, C435S252330, C536S023200
Reexamination Certificate
active
07052879
ABSTRACT:
The present invention features an isolated nucleic acid that includes a mutant DNA encoding aCandida rugosalipase, wherein the mutant DNA is 80% identical to a wild-type DNA encoding theCandida rugosalipase, and includes at least 12 (e.g., 13, 15, 17, or all) universal serine codons corresponding to CTG codons in the wild-type DNA. Each of the universal serine codons, independently, is TCT, TCC, TCA, TCG, AGT, or AGC. TheCandida rugosalipase can beCandida rugosalipase 1, 2, 3, 4, 5, or 8.
REFERENCES:
patent: WO 99/14338 (1999-03-01), None
Lilia Alberghina, et al.Cloning, Sequencing, and Expression of Candida rugosa Lipases. Methods in Enzymology 284:246-260, 1997. XP002067443.
Stefania Brocca, et al.Design, Total Synthesis, and functional overexpression of the Candida rugosa lip1 gene coding for a major industrial lipase. Protein Science 7(6):1415-1422, 1998. XP00929163.
Yoshiyuki Kawaguchi, et al.The codon CUG is read as serine in an asporogenic yeast Candida cylindracea. Nature 341(6238):164-166, Sep. 1989. XP000084053.
Shye-Jye Tang, et al.Recombinant Expression of the Candida rugosa lip4 Lipase in Escherichia coli.Protein Expression and Purification 20:308-313, 2000. XP002204904.
Guan-Chiun Lee, Shye-Jye Tang. Kuang-Hui Sun, Jei-Fu Shaw.Analysis of the Gene Family Encoding Lipases in Candida rugosa by Competitive Reverse Transcription-PCR. Applied and Environmental Microbiology, vol. 65, No. 9, Sep. 1999, pp. 3888-3895.
Shye-Jye Tang, Jei-Fu Shaw, Kuang-Hui Sun, Guang-Huan Sun, Terng-Yuan Chang, Ching-Kai Lin, Yuh-Chih Lo, and Guang-Chiun Lee.Recombinant Expression and Characterization of the Candida rugosa lip4 Lipase in Pichia pastoris: Comparison of Glycosylation. Activity, and Stability. Archives of Biochemistry and Biophysics. vol. 387, No. 1, Mar. 2001, pp. 93-98.
Rey-Chang Chang, Shu-Jen Chou, Jei-Fu Shaw.Multiple forms and functions of Candida rugosa lipase. Biotechnol. Appl. Biochem. vol. 19, 1994, pp. 93-97.
Liming Ge and Peter Rudolph.Simultaneous Introduction of Multiple Mutations Using Overlap Extension PCR. Biotechniques, vol. 22, No. 1, Jan. 1997, pp. 28-30.
Miroslaw Cygler and Joseph D. Schrag.Structure and conformational flexibility of Candida rugosa lipase. Biochimica et Biophysica Acta, vol. 1441, 1999. pp. 205-214.
Lilia Alberghina et al. “Protein Engineering of a Fungal Lipase”. Engineering with Lipases, Kluwer Academia Publishers, Netherlands, pp. 219-228, XP-002067509.
Marina Lotti et al. “Cloning and Analysis ofCandida cylindracealipase sequences”. Gene 124(1):45-55, 1993. XP-001084201.
Longhi et al. “Lipase I precursor (EC 3.1.1.3) (LIP1)”. Database Accession No. P20261-XP-002230351.
Lee Guan-Chiun
Shaw Jei-Fu
Tang Shye-Jye
Academia Sinica
Nashed Nashaat T.
LandOfFree
Recombinant Candida rugosa lipases does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Recombinant Candida rugosa lipases, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Recombinant Candida rugosa lipases will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3542181