Recombinant cage-like protein, method for producing the...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing organic compound containing a metal or atom other...

Reexamination Certificate

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C530S400000, C530S350000, C424S617000, C424S600000, C435S440000, C426S327000, C426S664000, C514S002600

Reexamination Certificate

active

07105323

ABSTRACT:
The present invention relates to a precious metal-recombinant apoferritin complex produced by recombination technique, wherein the precious metal is gold (Au) or platinum (Pt), and wherein the residues of glutamic acid and aspartic acid in a channel of apoferritin complex are substituted with small polar amino acid residues or/and noncharged amino acid residues, e.g., serine, or/and with basic amino acid residues, e.g., lysine. The substitution prevents a repulsive force due to electrostatic interaction between a metal ion, e.g., (AuCl4)−that has a negative charge and a negative amino acid residue of the apoferritin, and facilitates the capture of (AuCl4)−into holding portion in the channel of said metal-recombinant apoferritin complex. The captured (AuCl4)−is subsequently reduced to Au, and thus the gold-recombinant apoferritin complex is produced.

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