Recombinant biopesticide and method of use thereof

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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4352523, 4352525, 4353201, 435832, 424 931, 424 932, 424 934, 424 93461, 536 221, 536 231, 536 234, 536 237, 536 2371, C12P 2106, C12N 120, A01N 6300, A01N 6500

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055188974

ABSTRACT:
A recombinantly derived biopesticide active against Diptera includes cyanobacteria transformed with a plasmid containing a B. thuringiensis subsp. israelensis dipteracidal protein translationally fused to a strong, highly active native cyanobacterial regulatory gene sequence.

REFERENCES:
Delecluse et al., Deletion by In Vivo Recombination Shows that the 28-Kilodalton Cytolytic Polypeptide from Bacillus thuringiensis subsp. israelnesis Is Not Essential for Mosquitocidal Activity, Journal of Bacteriology, vol. 173, No. 11, pp. 3374-3381 (Jun. 1981).
Murphy et al., Purpose of the Project To Produce a Mosquitocidal Cyanobacterium That Can Successfully Compete in Mosquito Breeding Locales, May 6, 1991, Meeting of the American Society for Microbiology, Dallas, Texas.
Murphy et al., Nucleotide Sequence and Further Characterization of the Synechococcus sp. Strain PCC 7002 recA Gene: Complementation of a Cyanobacterial recA Mutation by the Escherichia coli recA Gene, Journal of Bacteriology, vol. 172, No. 2, pp. 967-976 (Feb. 1990).
Chungjatupornchai, Expression of the Mosquitocidal-Protein Genes of Bacillus thuringiensis subsp. israelensis and the Herbicide-Resistance Gene bar in Synechocystis PCC6803, Current Microbiology, vol. 21, pp. 283-288 (1990).
Angsuthanasombat et al., Biosynthesis of 130-Kioldalton Mosquito Larvicide in the Cyanobacterium Agmenellum quadruplicatum PR-6, Applied and Environmental Microbiology, vol. 55, No. 9, pp. 2428-2430 (Sep. 1989).
Hofte et al., Insecticidal Crystal Proteins of Bacillus thuringiensis, Microbiological Reviews, vol. 53, No. 2, pp. 242-255 (Jun. 1989).
Chilcott et al., Comparative Toxicity of Bacillus thuringiensis var. israelensis Crystal Proteins in vivo and in vitro, Journal of General Microbiology, 134, pp. 2551-2558 (1988).
Donovan et al., Molecular Characterization of a Gene Encoding a 72-Kilodalton Mosquito-Toxic Crystal Protein from Bacillus thuringiensis subsp. israelensis, Journal of Bacteriology, vol. 170, No. 10, pp. 4732-4738 (Oct. 1988).
Aronson et al., Bacillus thuringiensis and Related Insect Pathogens, Microbiological Reviews, vol. 50, No. 1, pp. 1-24 (Mar. 1986).
Buzby et al., Expression of the Escherichia coli lacZ Gene on a Plasmid Vector in a Cyanobacterium, Science, vol. 230, pp. 805-807 (Nov. 1985).
Angsuthanasombat et al. "Cloning and Expression of 130-kd mosquito-larvicidal .delta.-endotoxin gene of Bacillus thuringiensis var. Israelensis in Escherichia coli", Mol. Gen. Genet. (1987) 208:384-389.
Glover "Expression of cloned DNAs in E. coli plasmids" Gene Cloning (1984) pp. 110-126.

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