Recombinant bHLH-PAS/JHR polypeptide and its use to screen...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C435S320100, C435S069100, C435S325000, C435S348000, C435S252300, C435S007100, C435S254110, C536S023100, C536S023200, C536S023500, C536S024100, C536S024300

Reexamination Certificate

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06887661

ABSTRACT:
Promising groups of environmetally-safe insecticides consist of analogues of insect hormones, such as juvenile hormone, and antagonists of such hormones. The traditional bioassay approach for screening potential juvenile hormone analogs and antagonists is slow, expensive and inefficient. A recombinant bHLH-PAS-juvenile hormone receptor, isolated from the methoprene-tolerant locus onDrosophila, provides the basis of in vitro and in vivo binding assays that can be used to discover new juvenile hormone-type targeted insecticides. Moreover, the nucleotide sequence of theDrosophilabHLH-PAS/JHR polypeptide provides tools for isolating juvenile hormone receptor genes from other insect species.

REFERENCES:
patent: 6326165 (2001-12-01), Wilson et al.
Harvey et al. “HL01770, 5 prime HLDrosophila melanogasterhead BlueScript EST clone.” Database EST; Genbank Accession AA567788. dbEST entry; Aug. 21, 1997, updated Dec. 18, 1997.
Mak et al. “Expression of Functional Chicken Oviduct Progesterone Receptors in Yeast (Saccharomyces cerevisiae),” J. Biol. Chem., 264(36):21613-21618 (1989).
Mak et al. “Retinoid X Receptor Homodimers Function as Transcriptional Activators in Yeast,” Gene, 145: 129-133 (1994).
McDonnell et al. “Reconstitution of the Vitamin D-Responsive Osteocalcin Transcription Unit inSaccaromyces cerevisiae,”Molecular and Cellular Biology, 9(8):3517-3523 (1989).
Rowlands et al. “Aryl Hydrocarbon Receptor-Mediated Signal Transduction,” Critical Reviews in Toxicology, 27(2):109-134 (1997).
Rowlands et al. “Human Dioxin Receptor Chimera Transactivation in a Yeast Model System and Studies on Agonists and Antagonists,” Pharmacology & Toxicology, 76:328-333 (1995).
Shemshedini et al. “Evidence for a Juvenile Hormone Receptor Involved in Protein Synthesis inDrosopila melanogaster,” J. Biol. Chem., 265(4): 1913-1918 (1990).
Turner et al. “Molecular Analysis of the Methoprene-Tolerannt Gene Region ofDrosophila melanogaster,” Archives of Insect Biochemistry and Physiology, 30:133-147 (1995).
Whitelaw et al. “Identification of Transactivation and Repression Functions of the Dioxin Receptor and Its Basic Helix-loop-helix/PAS Partner Factor Arnt: Inducable Versus Constitutive Modes of Regulation,” Molecular and Cellular Biology, 14(12):8343-8355 (1994).
Wilson et al. “ADrosophila melanogasterMutant Resistant To A Chemical Analog Of Juvenile Hormone,” Developmental Biology, 118:190-201 (1986).
Yamaguchi et al. “Functional Analysis of Aryl Hydrocarbon Receptor Nuclear Translocator Interactions with Aryl Hydrocarbon Receptor in the Yeast Two-hybrid System,” Biochemical Pharmacology, 50(8): 1295-1302 (1995).
Yao et al. “Drosophila Ultraspiracle Modulates Esdysome Receptor Function Via Heterodimer Formation,” Cell, 71:63-72 (1992).
Zelzer et al. “The PAS Domain Confers Target Gene Specificity of Drosophila bHLH/PAS proteins,” Genes & Development, 11:2079-2089 (1997).
Shemshedini et al. “Resistance to Juvenile Hormone and an Insect Growth Regulator in Drosophila is Associated With an Altered Cytosolic Juvenile Hormone-Binding Protein,”Proc. Natl. Acad. Sci. USAArch., 87:2072-2076 (1990).
Kelley et al. “Restriction of P-Element Insertions at the Notch Locus ofDrosophila melanogaster,” Molecularand Cellular Biology, 7:1545-1548 (1987).
Ashok, M. et al., Insect juvenile hormone resistance gene homology with the bHLH-PAS family of transcriptional regulators, 1998 Proc. Natl. Acad. Sci., 95:2761-6.
Feyereisen, R., Juvenile hormone resistance:img id="CUSTOM-CHARACTER-00001" he="2.46mm" wi="0.68mm" file="US06887661-20050503-P00701.TIF" alt="custom character" img-content="character" img-format="tif" ?no PASaran!, 1998 Proc. Natl. Acad. Sci., 95:2725-6.
Minkoff, C. and Wilson, T.G., The Competitive Ability and Fitness Components of the Methoprene-tolerant (Met) Drosophila Mutant Resistance to Juvenile Hormone Analog Insecticides, 1992 Genetics Society of America, 91-7.
Shemshedini, L. and Wilson, T.G., Resistance to juvenile hormone and an insect growth regulator in Drosophila is associated with an altered cytosolic juvenile hormone-binding protein, 1990 Proc. Natl. Acad. Sci., 87:2072-6.
Turner, C. and Wilson, T.G., Molecular Analysis of the Methoprene-tolerant Gene Region ofDrosophila melanogaster,1995 Archives of Insect Biochemistry and Physiology, 30:133-47.

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