Recombinant .beta.-cell and uses thereof

Multicellular living organisms and unmodified parts thereof and – Method of making a transgenic nonhuman animal

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800 18, 800 22, 800 25, 424 9321, 435325, 435354, 435366, 435455, A01K 67027, C21N 1500, C21N 500, C21N 506, C21N 1563

Patent

active

061145998

ABSTRACT:
The present invention provides a recombinant, glucose-regulated insulin producing beta cell whose proliferation is controlled by tetracycline or a derivative thereof. The present invention also provides a method for treating a subject with diabetes using the recombinant beta cell of the present invention.

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patent: 5795790 (1998-08-01), Schinstine et al.
patent: 5935849 (1999-08-01), Schinstine et al.
D'Ambra et al. Regulation of insuling secretion from beta-cell lines derived from transgenic mice insulinomas resembles that of normal beta-cells. Endocrinology 126(6): 2815-2822, 1990.
Gossen et al. Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc. Natl. Acad. Sci. USA 89: 5547-5551, Jun. 1992.
Hammer et al. Spontaneous inflammatory disease in transgenic rats expressing HLA-B27 and human b2m: An animal model of HLA-B27-associated human disorders. Cell 63: 1099-1112. Nov. 1990.
Mullins et al. Expression of the DBA/2J Ren-2 gene in the adrenal gland of transgenic mice. EMBO J. 8(13): 4065-4072, 1989.
Mullins et al. Fulminant hypertension intransgenic rats harbouring the mouse Ren-2 gene. Nature 344: 541-544, Apr. 1990.
Taurog et al. HLA-B27 in inbred and non-inbred transgenic mice. J. of Immunol. 141(11): 4020-4023, Dec. 1988.
Wall, RJ Transgenic livestock: Progress and prospects for the future. Theriogenology 45: 57-68, 1996.

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