Recombinant .beta.-amylase

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor

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4353201, 536 232, C12N 120, C12N 1500, C07H 2104

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active

056886845

ABSTRACT:
A recombinant .beta.-amylase which is superior to the original recombinant .beta.-amylase in thermostability has been obtained by a site-directed mutagenesis with the recombinant .beta.-amylase gene coding 531 amino acid residues. Substitutions were MET.sub.181 of said enzyme with Leu, Ser.sub.291 with Ala, Ile.sub.293 with Val, Ser.sub.346 with Pro, Ser.sub.347 with Pro, Gln.sub.348 with Asp and Ala.sub.372 with Ser.

REFERENCES:
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patent: 5312739 (1994-05-01), Shaw
Oyo Toshitsu Kagaku, vol. 41, No. 2, pp. 261-271, 1994, Naohiro Yoshigi, et al., "Structure of Barley B-Amylase and Expression in Escherichia coli of cDNA".
Eur. J. Biochem., vol. 169, pp. 517-525, 1987, Martin Kreis, et al., "Primary Structure and Differential Expression of B-Amylase in Normal and Mutant Barleys".
Biosci. Biotech. Biochem, vol. 58, No. 6, pp. 1080-1086, 1994, Naohiro Yoshigi, et al., "Expression in Escherichia coli of cDNA Encoding Barley B-Amylase and Properties of Recombinant B-Amylase".
J. Biochem., vol. 115, pp. 47-51, 1994, Naohiro Yoshigi, et al., "PCR Cloning and Sequencing of the B-Amylase cDNA from Barley".
J. Biochem., vol. 118, pp. 562-567, 1995, Naohiro Yoshigi, et al., "Construction of a Plasmid Used for the Expression of a Sevenfold-Mutant Barley B-Amylase with Increased Thermostability in Escherichia coli and Properties of the Sevenfold-Mutant B-Amylase".

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