Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1985-06-03
1989-12-12
Martinell, James
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435 691, 4351721, 435202, 435235, 435832, 435839, 43525231, 935 31, 935 58, 935 74, C12N 1500, C12N 928, C12N 700, C12N 120, C12P 2100, C12R 107, C12R 1125
Patent
active
048867543
ABSTRACT:
A recombinant bacteriophage, a method for producing and selecting the recombinant bacteriophage and a method for heterologous cloning of DNA are disclosed. The recombinant bacteriophage is produced by ligating genetic fragments encoding a desired genetic trait with DNA from a bacteriophage, incubating with DNA from a second Bacillus microorganism prototrophic for a growth requirement, incubating with a host Bacillus auxotrophic for the growth requirement. Transformed host Bacillus are selected by growing the mixture on a growth medium which does not contain the growth requirement and determining the presence of the genetic trait. The recombinant bacteriophage containing the desired genetic trait is recovered from the host Bacillus by induction. Heterologous cloning can be accomplished by incubating a host Bacillus with the recombinant bacteriophage.
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Graham Richard S.
Yoneda Yuko
Young Frank E.
Lukacher Martin
Martinell James
The University of Rochester
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