Recombinant baculovirus with broad host range

Details Recombinant baculovirus with broad host range Recombinant baculovirus with broad host range

1995-04-06

1997-06-17

Guzo, David

Drug, bio-affecting and body treating compositions

Whole live micro-organism, cell, or virus containing

Genetically modified micro-organism, cell, or virus

435 691, 4351723, 4352351, 4353201, 435348, A01N 6300, C12N 1563, C12N 1586

Patent

active

056394543

ABSTRACT:
Insect control agents comprising a gene encoding a protein that affects the host range of baculoviruses. A unique gene encoding a protein of 25.7 kdal, p25.7, isolated from a baculovirus from the gypsy moth, Lymantria dispar nuclear polyhedrosis virus, that affects the host range of baculoviruses is disclosed. This "host range gene" allows a second baculovirus, Autographa californica nuclear polyhedrosis virus, to replicate in an insect cell line that will not normally support its replication. Methods for producing the insect control agents are included. A recombinant Autographa california nuclear polyhedrosis virus, in which the p25.7 gene has been inserted, was demonstrated to replicate in both a cell line and an insect species that the unaltered Autographa californica nuclear polyhedrosis virus does not infect. The recombinant Autographa californica nuclear polyhedrosis virus also shows increased virulence towards insects that are susceptible to infection by unaltered virus as demonstrated by a significant reduction in the dose of virus needed to kill 50% of the infected insect larvae (LD50).

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