Recombinant bacterial phytases and uses thereof

Details Recombinant bacterial phytases and uses thereof Recombinant bacterial phytases and uses thereof

2001-12-21

2008-11-18

Ramirez, Delia M (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

C435S019000, C435S069100, C435S320100, C435S252300, C435S254110, C435S252330, C435S254200, C435S348000, C435S325000, C424S094600, C426S656000, C530S350000, C536S023200

Reexamination Certificate

active

07452706

ABSTRACT:
A purified recombinant phytase enzyme derived fromEscherichia coliB. The enzyme has a molecular weight of about 47.1 kilodaltons and has phytase activity. The enzyme can be produced from native or recombinant host cells and can be used to aid in the digestion of phytate where desired. In particular, the phytase of the present invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients.

REFERENCES:
patent: 5284933 (1994-02-01), Döbeli et al.
patent: 5366736 (1994-11-01), Edwards, Jr.
patent: 5436156 (1995-07-01), Van Gorcom et al.
patent: 5492813 (1996-02-01), Eisenbeis et al.
patent: 5593963 (1997-01-01), Van Ooijen et al.
patent: 5750135 (1998-05-01), Schleicher et al.
patent: 5830696 (1998-11-01), Short
patent: 5830732 (1998-11-01), Mochizuki et al.
patent: 5866118 (1999-02-01), Berka et al.
patent: 5876997 (1999-03-01), Kretz
patent: 5939303 (1999-08-01), Cheng et al.
patent: 6039942 (2000-03-01), Lassen et al.
patent: 6190897 (2001-02-01), Kretz
patent: 2002/0136754 (2002-09-01), Short et al.
patent: 0 282 042 (1994-06-01), None
patent: 0 441 252 (1997-09-01), None
patent: 0 897 985 (1999-02-01), None
patent: 2316082 (1998-02-01), None
patent: 04 349881 (1992-12-01), None
patent: 09 504695 (1997-05-01), None
patent: 99086028 (1999-12-01), None
patent: WO-97/33976 (1997-09-01), None
patent: WO-98/44125 (1998-10-01), None
patent: WO 99/08539 (1999-02-01), None
patent: WO-00/58481 (2000-10-01), None
patent: WO 00/64247 (2000-11-01), None
patent: WO-00/64247 (2000-11-01), None
patent: WO-00/71728 (2000-11-01), None
patent: WO-01/90333 (2001-11-01), None
Branden et al., Introduction to Protein Structure, Garland Publishing Inc., New York, p. 247, 1991.
Stephen F. Atlschul, Warren Gish, Webb Miller, Eugene W. Myers and David J. Lipmann, “Basic Local Alignment Search Tool”, 1990, Academic Press Limited, J. Mol. Bio. vol. 215, pp. 403-410.
Henrik Brinch-Pedersen, Annette Olesen, Soren K. Rasmussen & Preben B. Holm, “Generation of Transgenic Wheat (Triticum aestivumL.) for Constitutive Accumulation of anAspergillusPhytase”, 2000, Molecular Breeding, pp. 195-206.
Janie Dassa, Christian Marck, and Paul L. Boquet, “The Complete Nucleotide Sequence of theEscherichia coliGeneappAReveals Significant Homology between pH 2.5 Acid Phosphatase and Glucose-1-Phosphatase”, Sep. 1990, Journal of Bacteriology, vol. 172, No. 9, pp. 5497-5500.
J. Dvorakova, “Phytase: Sources, Preparation and Exploitation”, 1998, Folia Microbiol, vol. 43(4), pp. 323-338.
Lori Giver, Anne Gershenson, Per-Ola Freskgard, and Frances H. Arnold, “Directed Evolution of a Thermostable Esterase”, Oct. 1998, National Academy of Sciences, vol. 95, pp. 12809-12813.
R. Greiner, U. Konietzny, and Kl..-D. Jany, “Purification and Characterization of Two Phytases fromEscherichia coli”, May 15, 1993, Archives of Biochemistry and Biophysics, vol. 303, No. 1, pp. 107-113.
M. Lehmann, L. Pasamontes, s. F. Lassen, M. Wyss, “The Consensus Concept for Thermostability Engineering of Proteins”, 2000, Biochimica et Biophysica Acta, vol. 1543, pp. 408-415.
Lutz Jermutus, Michel Tessier, Luis Pasamontes, Adolphus P.G.M. van Loon, and Martin Lehmann, “Structure-based Chimeric Enzymes as an Alternative to Directed Enzyme Evolution: Phytase as a Test Case”, 2001, Journal of Biotechnology vol. 85, pp. 15-24.
William R. Pearson and David J. Lipman, “Improved Tools for Biological Sequence Comparison”, Apr. 1988, National Academy of Sciences, vol. 85, pp. 2444-2448.
Jan Pen, Theo C. Verwoerd, Peter A. vanParidon, Rob F. Beudeker, Peter J.M. van den Elzen,Kees Geerse, Jan D. van der Klis, Hans A. J. Versteegh, Albert J.J. van Ooyen and Andre' Hoekema, “Phytase-containing Transgenic Seed as a Novel Feed Additive for Improved Phosphorus Utilization”, Jul. 1993, Bio/Technology vol. 11, pp. 79-82.
Eric Rodriguez, Yanming Han, and Xin Gen Lei, “Cloning, Sequencing, and Expression of anEscherichia coliAcid Phosphatase/Phytase Gene (appA2) Isolated from Pig Colon”, 1999, Biochemical and Biophysical Research Communications, vol. 257, pp. 117-123.
J. Rozas and R. Rozas, “DnaSP, DNA Sequence Polymorphism: An Interactive Program for Estimating Population Genetics Parameters from DNA Sequence Data”, 1995, Cabios, vol. 11 No. 6, pp. 61-625.
Andrea Tomschy, Michel Tessier, Markus Wyss, Roland Brugger, Clemens Broger, Line Schnoebelen, Adolphus P.G.M. van Loon, and Luis Pasamontes, “Optimization of the Catalytic Properties ofAspergillus-fumigatusPhytase Based on the Three-dimensional Structure”, 2000, Protein Science, pp. 1304-1311.
T. C. Verwoerd, A. Hoekema, P. A. van Paridon, A.J.J. van Ooyen, & J. Pen, “Phytase-enriched Transgenic Seeds as a Novel Feed Additive”, 1993, Med. Fac. Landbouww. Univ. Gent. No. 58/4a, pp. 1719-1721.
Costantino Vetriani, Dennis L. Maeder, Nicola tolliday, Kitty S.-P. Yip, Timothy J. Stillman, K. Linda Britton, David W. Rice, Horst H. Klump, and Frank T. Robb, “Protein Thermostability Above 100° C: A Key Role for Ionic Interactions”, Oct. 1998, National Academy of Sciences, vol. 95, pp. 12300-12305.
Gunnar von Heijne, A New Method for Predicting Signal Sequence Cleavage Sites, 1986, Research Group for Theoretical Biophysics, pp. 4683-4690.
Markus Wyss, Roland Brugger, Alexandra Kronenberger, Roland Remy, Rachel Fimbel, Gottfried Oesterhelt, Martin Lehmann, and Adolphus P.G.M. van Loon, Feb. 1999, Applied and Environmental Microbiology, vol. 65, No. 2, pp. 367-373.
GenBank Database accession No. A02249, Apr. 1996.
Golovan et al., Characterization and overproduction of theEscherichia coli appAencoded bifunctional enzyme that exhibits both phytase and acid phosphatase activities,Canadian Journal of Microbiology, vol. 46, No. 1, pp. 59-71, Jan. 2000.
Bork, et al., “Powers and Pitfalls in Sequence Analysis: The 70% Hurdle”,Genome Research, pp. 398-400, vol. 10, 2000.
Broun, et al., “Catalytic Plasticity of Fatty Acid Modification Enzymes Underlying Chemical Diversity of Plant Lipids”,Science, vol. 282, pp. 1315-1317, Nov. 13, 1998.
Van de Loo, et al., “An oleate 12-hydroxylase fromRicinus communisL. is a fatty acyl desaturase homolog”,Proc. Natl. Acad. Sci., vol. 2, pp. 6743-6747, 1995.
Seffernick, et al., “Melamine Deaminase and Atrazine Chlorohydrolase: 98 percent Identical but Functionally Different”,Journal of Bacteriology, vol. 183, No. 8; pp. 2405-1410, Apr. 2001.
Greiner et al., Archives of Biochemistry and Biophysics 34(2):201-206 (1997).
Institute of Applied Environmental Economics (TME) of the Netherlands, “Use of Phytase in Pig and Poultry Feed to Reduce Phosphorus Excretion,” (1995).
Bae et al., Geneseq accession No. ABK12514, Dec. 15, 1999.
Forsberg et al., Geneseq accession No. AAC68296, Nov. 2, 2000.
Forsberg et al., Geneseq accession No. AAC68299, Nov. 2, 2000.
International Search Report mailed on Jul. 27, 2004, for PCT patent application No. PCT/US02/16482 filed on May 24, 2002, 9 pages.
Ostanin et al., GenBank accession No. L03371 (1992).
Ostanin et al., J Biol Chem 267(32):22830-22836 (1992).
Berka et al., Applied and Environ. Biol. (1998) 64:4423-4427.
Bouquet et al., J. of Bacteriology (1987) 169:1663-1669.
Casey and Walsh, J. Biotechnol. (2004) 110:313-322.
Delagrave et al., Protein Eng. (1993) 6:327-331.
Delagrave et al., Nature Biotech. (1993) 11:1548-1552.
Kerovuo et al., Applied and Environ. Biol. (1998) 64:2079-2085.
Lim et al., Nature Structural Biology (2000) 7:108-113.
NCBI GenBank entry AAB96873 phytase, Jan. 1998.
NCBI GenBank entry AAA16898 phytase, Mar. 1994.
NCBI GenBank entry AAB26466 phytase, Oct. 1993.
NCBI GenBank entry AAB96871 phytase, Jan. 1998.
Oh et al., Abstracts of the General Meeting of the American Society for Microbiology (2000) 100:499-500.
Rodriguez et al., Archives of Biochemistry and Biophysics (1999) 365:262-267.
Rodriguez et al., Archives of Biochemistry and

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