Recombinant bacteria expressing functional R76 mammalian recepto

Details Recombinant bacteria expressing functional R76 mammalian recepto Recombinant bacteria expressing functional R76 mammalian recepto

1991-03-25

1993-09-07

Hill, Jr., Robert J.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435 691, 435 697, 4353201, 436501, C12N 1562, C12N 1563

Patent

active

052428222

ABSTRACT:
The invention relates to a vector capable of being replicated in cultures of unicellular organisms, this vector containing a gene coding for a eucaryotic protein having the biological activity of a membrane receptor and interacting with a regulatory protein--called the G protein--able to bind molecules of guanosine triphosphate (GTP). The invention also relates to cell organisms transformed by the above vectors. It also relates to procedures for the detection of the capacity of a molecule to behave as a ligand for a receptor and a procedure for studying the affinity of a receptor for a ligand as well as a kit for detecting the possible affinity of a ligand for a receptor.

REFERENCES:
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