Recombinant bacillus proteases and uses thereof

Details Recombinant bacillus proteases and uses thereof Recombinant bacillus proteases and uses thereof

2006-07-25

2006-07-25

Prouty, Rebecca E. (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

C435S069100, C435S221000, C435S222000, C435S320100, C435S252310, C536S024100, C536S023200

Reexamination Certificate

active

07081359

ABSTRACT:
The present invention provides genetically engineeredBacillusstrains that can secrete large amount ofBacillusproteases in the extracellular culture medium. More particularly, this invention relates to a process of producing recombinant protease molecules ofBacillusorigin in aBacillus subtilisstrain 168, utilizing a strong prophage promoter.

REFERENCES:
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Jacobs et al. Cloning, sequencing and expression of subtilisin Carlsberg fromBacillus licheniformis. Nucleic Acids Res. Dec. 20, 1985;13(24):8913-26.
Leung and Errington, Characterization of an insertion in the phage phi 105 genome that blocks hostBacillus subtilislysis and provides strong expression of heterologous genes. Gene. Feb 27, 1995;154(1):1-6.
Thornewell et al. An efficient expression and secretion system based onBacillus subtilisphage phi 105 and its use for the production ofB. cereusbeta-lactamase I. Gene, Oct. 29, 1993;133(1):47-53.
van der Laan et al. Cloning, characterization, and multiple chromosomal integration of aBacillusalkaline protease gene. Appl Environ Microbiol. Apr. 1991;57(4):901-9.
Wells et al. Cloning, sequencing, and secretion ofBacillus amyloliquefacienssubtilisin inBacillus subtilis. Nucleic Acids Res. Nov. 25, 1983;11(22):7911-25.
Zaghloul et al. High level of expression and stability of the cloned alkaline protease (aprA) gene inBacillus subtilis. Enzyme Microb Technol. Jun. 1994;16(6):534-7.

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