Recombinant Bacillus phytases and uses thereof

Details Recombinant Bacillus phytases and uses thereof Recombinant Bacillus phytases and uses thereof

2002-11-19

2008-03-04

Baum, Stuart F. (Department: 1638)

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

C800S298000, C800S306000, C800S322000, C800S293000, C800S292000, C800S294000, C435S320100, C435S419000, C435S430000

Reexamination Certificate

active

07339091

ABSTRACT:
In this invention, two phytase genes from two generally-regarded-as-safe microorganisms,Bacillus licheniformisandBacillus subtilis168, were cloned and characterized. A process for phytase enzyme over-expression and purification was also developed. The enzymes have molecular weight of about 48 kilodaltons and showed extracellular phytate-hydrolyzing activities. The recombinant enzyme can be used to enhance phytase utilization in various commercial areas, including preparation of animal feed and transgenic plants that have increased growth rates for maturity, flowering and fruiting.

REFERENCES:
patent: 5593963 (1997-01-01), Van Ooijen et al.
Wyss et al (1999, Applied and Environmental Microbiology 65(2):367-373).
Fourgoux-Nicol et al (1999, Plant Molecular Biology 40 :857-872).
Kerovuo et al (1998, Applied and Environmental Microbiology 64(6):2079-2085).
Berka, R.M., Rey M.W., Brown K.M., Byun T, Klotz A.V. (1998) Molecular characterization and expression of a phytase gene from the thermophilic fungusFusarium venenatum. Applied and Environmental Microbiology.64(11), 4423-4427.
Craxton A., Caffrey J.J., Burkhart W., Safrany S.T., Shears S.B. (1997) Molecular cloning and expression of a rat hepatic multiple inositol polyphosphate phosphatase.Biochemistry Journal, 328, 75-81.
Dassa J., Marck C. and Boquet P.L. (1990) The complete Nucleotide Sequence of theEscherichia coligeneappAreveals significant homology between pH 2.5 acid phosphatase and glucose-1-phosphtase,Journal of Bacteriology 172(9), 5497-5500.
Engelen A.J., Heeft F.C., Randsdorp P.H.G., Smit E.L.C. (1994) Simple and Rapid Determination of Phytase Activity.Journal of AOAC International 77(3), 760-764.
Greiner R., Haller, E., Konietzny U., Jany K.D. (1997) Purification and characterization of a phytase fromKlebsiella terrigena. Archives of Biochemistry and Biophysics.341(2), 201-206.
Han Y. Wilson, D.B., Lei X.G. (1999) Expression of anAsperigillus nigerphytase gene (phyA) inSaccharomyces cerevisiae. Applied and Environmental Microbiology.65(5), 1915-1928.
Han Y & Lei X.G. (1999) Role of glycosylation in the functional expression of anAsperigillusphytase (phyA) inPichia pastoris. Archives of Biochemistry and Biophysics.364(1), 83-90.
Hiei Y, Ohta S, Komari T, Kumashiro T. Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA. Plant J, 1994, 6:271-282.
Höfgen R, Willimitzer L (1988) Storage of competent cells for Agrobacterium transformation. Nucleic Acids Res 16:9877.
Kerovuo J., Laurarus M., Nurminen P., Kalkkinen N., Apajalahti J. (1998) Isolation, characterization, molecular gene cloning, and sequencing of a novel phytase fromBacillus subtilis, Applied and Environmental Microbiology.64(6), 2079-2085.
Kim Y.O., Lee J.K., Kim H.K., Yu J.H., Oh T.K. (1998) Cloning of the thermostable phytase gene (phy) fromBacillussp. DS11 and its overexpression inEscherichia coli. FEMS Microbiology Letters, 162, 182-191.
Leung Y.C. & Erington J. (1995) Characterization of an insertion in the phage f105 genome that blocks hostBacillus subtilislysis and provides strong expression of heterologous genes.Gene, 154, 1-6.
Maugenest S., Martinez I & Lescure A. (1997) Cloning and characterization of a cDNA encoding a maize seedling phytase.Biochemistry Journal, 322, 511-517.
Mayer A.F., Hellmuth, K., Schlieker H., Ulibarri R.L., Oertel S., Dahlems U., Strasser A.W.M. Strasser, Loon A.P.G.M. (1998) An expression eyetem matures: A highly efficient and cost-effective process for phytase production by recombinant strains ofHansenula polymorpha. Biotechnology and Bioengineering.63(3), 373-381.
Osburne M.S., Craig R. J. and Rothstein D. M. (1985) Thermoniducible transcription system forBacillus subtilisthat utilizes control elements from temperate phage f 105.J. of Bacteriology 16, 1101-1108.
Ostanin K., Harms E.H., Stevis P.E., Kuciel R., Zhou M.M., Van Etten R.L. (1992) Overpression, site-directed mutagenesis, and mechanism ofEscherichia coliand phosphatase.Journal of Bacteriology, 267(32), 22830-22836.
Pasamontes L, Haiker M, Wyss M, Tessier M, Loon A.P.G. (1997) Gene cloning, purification, and characterization of a heat-stable phytase from the fungusAspergillus fumigantus. Applied and Environmental Microbiology.63(5), 1696-1700.
Pen J. Verwored T.C., Hoekema A. (1993) Phytase-containing transgenic seeds as novel feed additive for improved phosphorus utilization.Bio/Technology, 11, 811-814.
Phillippy B.Q. and Mullaney E.J. (1997) Expression of anAspergillus nigerphytase (phyA) inEscherichia coli. Journal of Agricultural Food Chemistry, 45, 3337-3342.
Powar V.K. and Jagannathan V. (1982) Purification and properties of Phytase-Specific Phosphatase fromBacillus substilis. Journal of Bacteriology.151(3), 1102-1108.
Rashid H, Yokoi S, Toriyama K, Hinata K (1996) Transgenic plant production mediated byAgrobacteriumin Indica rice. Plant cell Rep, 15:727-730.
Reddy N. R., Pierson M. D., Sathe S. K. & Salunkhe D. K. (1989) Phytases in legumes and cereals.CRC Press, Inc., Boca Raton, Florida.
Rodriguez E., Mullaney E.J., Lei X.G. (2000) Expression of theAspergillus fumigatusphytase gene inPichia pastorisand characterization of the recombinant enzyme.Biochemical and Biophysical Research Communications, 268, 373-378.
Rodriguez E., Han Y. and Lei X.G. (1999) Cloning, Sequencing, and Expression of anEscherichia coliAcid Phosphatase/phytase Gene (appA2) Isolated from Pig Colon.Biochemical and Biophysical Research Communications, 257, 117-123.
Thornwwell S. J., Ease A.K., Errington J. (1993) An efficient expression and secretion system based onBacillus subtilisphage f105 and its use for the production ofB. cereusβ-lactarnase I.Gene, 133, 47-53.
Ullah A.H., Cummins B.J. (1988)Aspergillus ficuumextracellular pH 6.0 optimum acid phosphatase: purification, N-terminal amino acid sequence, and biochemical characterization.Preparative Biochemistry, 18(1), 37-65.
Ullah A.H.J., Sethumadhavan K., Mullaney E.J. (1999) Characterization of recombinant fungal phytase (phyA) expressed in tobacco leaves.Biochemical and Biophysical Research Communications, 264, 201-206.
Chu C-C, Wang J-J, Sun J-S, Xu Z, Yin G-C, Zhu Z-Y, Bi F-Y (1975), Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Sci Sin, 18:659-668.

Affiliated with

Also associated with

Rating

Recombinant Bacillus phytases and uses thereof does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Recombinant Bacillus phytases and uses thereof, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Recombinant Bacillus phytases and uses thereof will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3963535