Recombinant antibody-enzyme fusion proteins

Details Recombinant antibody-enzyme fusion proteins Recombinant antibody-enzyme fusion proteins

1998-04-30

2000-10-17

Eyler, Yvonne

Drug, bio-affecting and body treating compositions

Antigen, epitope, or other immunospecific immunoeffector

Fusion protein or fusion polypeptide

424 943, 514 12, 435188, 5303881, 5303882, 5303388, 53038885, 530866, 530867, 530324, A61K 39395, A61K 4716, C12N 996

Patent

active

061327220

ABSTRACT:
The invention is related to recombinantly produced fusion polypeptides comprising antibody V.sub.H and V.sub.L sequences operatively linked to a .beta.-lactamase for use in the delivery of cytotoxic drugs to tumor cells.

REFERENCES:
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Goshorn et al., "Genetic Construction, Expression, and Characterization of a Single Chain Anti-Carcinoma Antibody Fused to .beta.-Lactamase," Cancer Res. 53:2123-2127 (May 1, 1993).
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Bosslet et al., "Tumor-Selective Prodrug Activation by Fusion Protein-Mediated Catalysis," Cancer Res. 54:2151-2159 (Apr. 15, 1994).
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Rodrigues et al., "Development of a Humanized Disulfide-Stabilized Anti-p185.sup.HER2 Fv-.beta.-Lactamase Fusion Protein for Activation of a Cephalosporin Doxorubicin Prodrug," Cancer Res. 55:63-70 (Jan. 1, 1995).
Svensson et al., "In Vitro and In Vivo Activities of a Doxorubicin Prodrug in Combination with Monoclonal Antibody .beta.-Lactamase Conjugates," Cancer Res. 55:2357-2365 (Jun. 1, 1995).
Kerr et al., "Regressions and Cures of Melanoma Xenografts Following Treatment with Monoclonal Antibody .beta.-Lactamase Conjugates in Combination with Anticancer Prodrugs," Cancer Res. 55:3558-3563 (Aug. 15, 1995).
Siemers et al., "Modifying the Specificity and Activity of the Enterobacter cloacae P99 .beta.-Lactamase by Mutagenesis within an M13 Phage Vector," Biochem. 35:2104-2111 (1996).
Siemers et al., "Construction, Expression, and Activities of L49-sFv-.beta.-Lactamase, a Single-Chain Antibody Fusion Protein for Anticancer Prodrug Activation," Bioconjugate Chem. 8:510-519 (1997).

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