Recombinant antibody-enzyme fusion proteins

Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Fusion protein or fusion polypeptide

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Details

424 943, 514 12, 435188, 5303881, 5303882, 5303388, 53038885, 530866, 530867, 530324, A61K 39395, A61K 4716, C12N 996

Patent

active

061327220

ABSTRACT:
The invention is related to recombinantly produced fusion polypeptides comprising antibody V.sub.H and V.sub.L sequences operatively linked to a .beta.-lactamase for use in the delivery of cytotoxic drugs to tumor cells.

REFERENCES:
patent: 4975278 (1990-12-01), Senter et al.
Yeh et al., "Cell Surface Antigens of Human Melanoma identified by Monoclonal Antibody," Proc. Natl. Acad. Sci. USA 76:2927-2931 (Jun., 1979).
Woodbury et al., "Identification of a Cell Surface Protein, p97, in Human Melanomas and Certain Other Neoplasms," Proc. Natl. Acad. Sci. USA 77:2183-2187 (Apr., 1980).
Brown et al., "Quantitative Analysis of Melanoma-Associated Antigen p97 in Normal and neoplastic Tissues," Proc. Natl. Acad. Sci. USA 78:539-543 (Jan., 1981).
Brown et al., "Structural characterization of Human Melanoma-Associated Antigen p97 with Monoclonal Antibodies," J. Immunol. 127:539-546 (Aug., 1981).
Bosslet et al., "Molecular and Functional Characterisation of a Fusion Protein Suited for Tumour Specific Prodrug Activation," Br. J. Cancer 65:234-238 (1992).
Goshorn et al., "Genetic Construction, Expression, and Characterization of a Single Chain Anti-Carcinoma Antibody Fused to .beta.-Lactamase," Cancer Res. 53:2123-2127 (May 1, 1993).
Senter et al., "Generation of Cytotoxic Agents by Targeted Enzymes," Bioconjugate Chem. 4:3-9 (1993).
DeSutter et al., "A bifunctional Murine::Human Chimeric Antibody with One Antigen-Binding Arm Replaced by Bacterial .beta.-Lactamase," Mol. Immunol. 31:261-267 (1994).
Bosslet et al., "Tumor-Selective Prodrug Activation by Fusion Protein-Mediated Catalysis," Cancer Res. 54:2151-2159 (Apr. 15, 1994).
Wallace et al., "Intratumoral Generation of 5-Fluorouracil Mediated by an Antibody-Cytosine Deaminase Conjugate in Combination with 5-Fluorocytosine," Cancer Res. 54:2719-2723 (May 15, 1994).
Jungheim and Shepherd, "Design of Antitumor Prodrugs: Substrates for Antibody Targeted Enzymes," Chem. Rev. 94:1553-1566 (1994).
Bagshawe et al., "Antibody Directed Enzyme Prodrug Therapy (ADEPT)," Ann. Oncol. 5:897-891 (1994).
Rodrigues et al., "Development of a Humanized Disulfide-Stabilized Anti-p185.sup.HER2 Fv-.beta.-Lactamase Fusion Protein for Activation of a Cephalosporin Doxorubicin Prodrug," Cancer Res. 55:63-70 (Jan. 1, 1995).
Svensson et al., "In Vitro and In Vivo Activities of a Doxorubicin Prodrug in Combination with Monoclonal Antibody .beta.-Lactamase Conjugates," Cancer Res. 55:2357-2365 (Jun. 1, 1995).
Kerr et al., "Regressions and Cures of Melanoma Xenografts Following Treatment with Monoclonal Antibody .beta.-Lactamase Conjugates in Combination with Anticancer Prodrugs," Cancer Res. 55:3558-3563 (Aug. 15, 1995).
Siemers et al., "Modifying the Specificity and Activity of the Enterobacter cloacae P99 .beta.-Lactamase by Mutagenesis within an M13 Phage Vector," Biochem. 35:2104-2111 (1996).
Siemers et al., "Construction, Expression, and Activities of L49-sFv-.beta.-Lactamase, a Single-Chain Antibody Fusion Protein for Anticancer Prodrug Activation," Bioconjugate Chem. 8:510-519 (1997).

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