Recombinant antibody composition

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...

Reexamination Certificate

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C530S387100

Reexamination Certificate

active

07923538

ABSTRACT:
The present invention relates to a recombinant antibody composition having higher complement-dependent cytotoxic activity than a human IgG1 antibody and a human IgG3 antibody, wherein a polypeptide comprising a CH2 domain in the Fc region of a human IgG1 antibody is replaced by a polypeptide comprising an amino acid sequence which corresponds to the same position of a human IgG3 antibody indicated by the EU index as in Kabat, et al.; a DNA encoding the antibody molecule or a heavy chain constant region of the antibody molecule contained in the recombinant antibody composition; a transformant obtainable by introducing the recombinant vector into a host cell; a process for producing the recombinant antibody composition using the transformant; and a medicament comprising the recombinant antibody composition as an active ingredient.

REFERENCES:
patent: 6737056 (2004-05-01), Presta
patent: 7297775 (2007-11-01), Idusogie et al.
patent: 2003/0158389 (2003-08-01), Idusogie et al.
patent: 2005/0208519 (2005-09-01), Liew et al.
patent: 2007/0111281 (2007-05-01), Sondermann et al.
patent: 1443961 (2004-08-01), None
patent: 02/056910 (2002-07-01), None
patent: 03-035835 (2003-05-01), None
patent: 2006-020114 (2006-02-01), None
Kabat, et al. Sequences of proteins of immunological interest. 1987. 4thedition. pp. 307-308.
Tao, M., Smith, R.I.F., and Morrison, S.L. Structural features of human immunoglobulin G that determine isotype-specific differences in complement activation. Journal of Experimental Medicine, 1993. vol. 178, pp. 661-667.
Shinkawa, Nakamura, Yamane, Shoji-Hosaka, Kanda, Sakurada, Uchida, Anazawa, Satoh, Yamasaki, Hanai, and Shitara. The absence of fucose but not the presence of galactose or bisecting N-acetylglucosamine of human IgG1 complex-type oligosaccharides shows the critical role of enhancing ADCC. Journal of Biological Chemistry, 2003. vol. 278, p. 3466-73.
Kushihata, F., Watanabe, J., Mulder, A., Claas, F., and Scornik, J.C. Human leukocyte antigen antibodies and human complement activation: role of IgG subclass, specificity, and cytotoxic potential. Transplantation, 2004. vol. 78, pp. 995-1001.
Norderhaug, L., Brekke, O.H., Bremnes, B., Sandin, R., Aase, A., Michaelsen, T.E., and Sandlie, I. Chimeric mouse human IgG3 antibodies with an IgG4-like hinge region induce complement-mediated lysis more efficiently than IgG3 with normal hinge. European Journal of Immunology, 1991. vol. 21, pp. 2379-2384.
Michaelsen, T.E., Aase, A., Westby, C., and Sandlie, I. Enhancement of complement activation and cytolysis of human IgG3 by deletion of hinge exons. Scandinavian Journal of Immunology, 1990. vol. 32, pp. 517-528.
Amaral et al (Biosep., 10:139-143, 2002).
Teeling et al (Blood, 104:1793-1800, 2004).
Brekke O.H et al., Human IgG3 can adopt the disulfide bond pattern characteristic for IgG1 without resembling it in complement mediated cell lysis, Mol. Immunol., 1993, vol. 30, p. 1419-1425.
Yamane-Ohnuki N. et al., Establishment of FUT8 knockout Chinese hamster ovary cells: an ideal host cell line for producing completely defucosylated antibodies with enhancedantiboby-dependent cellular cytotoxicity, Biotechnol. Bioeng., 2004, vol. 87, p. 614-622.
Akira Okazaki et al., “Fucose Kesshitsu ni yoru Kotai Efector Kassei no Kojo”, Seikagaku, 2005 Nen, 1 GAtsu, vol. 77, pp. 45 to 50.
Robert L. Shields, et al.; “Lack of Fucose on Human IgG1 N-Linked Oligosaccharide Improves Binding to Human FcyRIII and Antibody-dependent Cellular Toxicity”; May 2002; Journal of Biological Chemistry; pp. 26733-26740; vol. 277, No. 30.
Niwa, Rinpei et al. “Enhancement of the Antibody-Dependent Cellular Cytotoxicity of Low-Fucose IgG1 is Independent of FcγRIIIa Functional Polymorphism”, Clinical Cancer Research, The American Association for Cancer Research, 2004, 10(18): 6248-6255. XP002354666.
Medesan, Corneliu et al. “Delineation of the Amino Acid Residues Involved in Transcytosis and Catabolism of Mouse IgG1”, Journal of Immunology, 1997, 158(5): 2211-2217. XP002532767.

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