Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...
Reexamination Certificate
2008-03-25
2008-03-25
Guzo, David (Department: 1636)
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
Introduction of a polynucleotide molecule into or...
C435S320100, C435S455000
Reexamination Certificate
active
07348178
ABSTRACT:
The present invention relates to a novel Ad based packaging system that can be used for incorporation of heterologous DNA into infectious but replication defective viral particles. The components of the invention include an “artificial genome”, i.e., a recombinant vector which contains elements that function as adenovirus replication and packaging signals flanking an intervening DNA sequence. The elements may comprise the minimum genomic Ad sequences required to direct replication of heterologous DNA and packaging into viral particles. The system also includes a means for expressing complementing helper functions to provide in trans viral proteins required for replication and packaging of recombinant viral vectors, but without contaminating the stock of recombinant, trans-packaged viral particles.
REFERENCES:
patent: 5882877 (1999-03-01), Gregory et al.
Grable et al. “cis and trans Requirements for the Selective Packaging of Adenovirus Type 5 DNA”, J. of Virol., Feb. 1992, vol. 66, No. 2, pp. 723-731.
Stratford-Perricaulet et al. “Gene Transfer into Animals: The Promise of Adenoviruses”, Human Gene Transfer, vol. 219, 1991, pp. 51-61.
Mitani et al., “Rescue, propagation, and partial purification of a helper virus-dependent adenovirus vector”, PNAS, vol. 92, pp. 3854-3858, Apr. 1995.
Bhatti, A. and Weber, J., 1979, “Protease of Adenovirus Type 2: Partial Characterization”, Virology 96:478-485.
Tibbetts, C. and Glam, C-Z., 1979, “In Vitro Association of Empty Adenovirus Capsids with Double-Stranded DNA”, J. Virol. 32:995-1005.
Stillman, B. et al., 1981, “Identifiation of the Gene and mRNA for the Adenovirus Terminal Protein Precursor”, Cell 23:497-508.
Saito, I. et al., 1985, “Construction of Nondefective Adenovirus Type 5 Bearing a 2.8-Kilobase Hepatitis B Virus DNA Near the Right End of its Genome”, J. Virol. 54:711-719.
Haj-Ahmad, Y. and Graham, F., 1986, “Development of a Helper-Independent Human Adenovirus Vector and Its Use in the Transfer of the Herpes Simplex Virus Thymidine Kinase Gene”, J. Virol. 57:267-274.
Kosturko, L. and Vanech, M., 1986/87, “In Vitro Encapsidation of Plasmid DNA into Human Adenovirus Empty Capsids”, Virus Research 6:123-132.
Ghosh-Choudhury, G. et al., 1987, “Protein IX, a Minor Component of the Human Adenovirus Capsid, is Essential for the Packaging of Full Length Genomes”, EMBO J. 6:1733-1739.
Hearing, P. et al., 1987, “Identification of a Repeated Sequence Element Required for Efficient Encapsidation of the Adenovirus Type 5 Chromosome”, J. Virol. 61:2555-2558.
Prevec, L. et al., 1990, “A Recombinant Human Adenovirus Vaccine Against Rabies”, J. Infect. Dis. 161:27-30.
Eloit, M. et al., 1990, “Construction of a Defective Adenovirus Vector Expressing the Pseudorabies Virus Glycoprotein gp50 and Its Use as a Live Vaccine”, J. Gen. Virol. 71:2425-2431.
Vernon, S. et al., 1991, “Ultrastructural Characterization of Human Immunodeficiency Virus Type 1 Gag-Containing Particles Assembled in a Recombinant Adenovirus Vector System”, J. Gen. Virol. 72:1243-1251.
Grable, M. and Hearing, P., 1992, “cis and trans Requirements for the Selective Packaging of Adenovirus Type 5 DNA”, J. Virol. 66:723-731.
Lucito, R. and Schneider, R., 1992, “Hepatitis B Virus X Protein Activates Transcription Factor NF-xB Without a Requirement for Protein Kinase C”, J. Virol. 66:983-991.
Berkner, K., 1992, “Expression of Heterologous Sequences in Adenoviral Vectors”, Curr. Topics in Microbiol. Immunol. 158:39-66.
Rosenfeld, M. et al., 1991, “Adenovirus-Mediated Transfer of a Recombinant α1-Antitrypsin Gene to the Lung Epithelium in Vivo”, Science 252:431-434.
Wilkinson, G. and Akrigg, A., 1992, “Constitutive and Enhanced Expression from the CMV Major IE Promoter in a Defective Adenovirus Vector”, Nucl. Acids Res. 20:2233-2239.
Le Gal La Salle, G. et al., 1993, “An Adenovirus Vector for Gene Transfer into Neurons and Glia in the Brain”, Science 259:988-990.
Zabner, J. et al., 1993, “Adenovirus-Mediated Gene Transfer Transiently Corrects the Chloride Transport Defect in Nasal Epithelia of Patients with Cystic Fibrosis”, Cell 75:207-216.
Guzo David
Jones Day
New York University Medical Center
LandOfFree
Recombinant adenoviral vector system does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Recombinant adenoviral vector system, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Recombinant adenoviral vector system will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2791062