Recombinant adeno-associated virus vectors

Details Recombinant adeno-associated virus vectors Recombinant adeno-associated virus vectors

1995-06-02

1998-07-14

Mosher, Mary E.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

4352351, 4353201, 935 32, 935 57, C12N 1509, C12N 1586, C12N 1564

Patent

active

057802807

ABSTRACT:
Simplified methods to produce recombinant adeno-associated virus (rAAV) vectors are described. The methods involve the use of chimeric plasmids which incorporate the Epstein Barr nuclear antigen (EBNA) gene, the latent origin of replication of Epstein Barr virus (oriP), and a rAAV genome. The chimeric plasmids themselves are also a part of the present invention. These plasmids are maintained as multicopy extra-chromosomal elements in cells, such as human 293 cells. Permanent cell lines carrying these EBV/AAV plasmids are induced to produce large amounts of rAAV upon addition of wild-type, adeno-associated virus helper functions. Vectors produced in this manner are capable of transducing exogenous genes into other human cell lines and exhibit the attributes of viral elements produced by conventional methods.

REFERENCES:
patent: 4686186 (1987-08-01), Sugden
patent: 4797368 (1989-01-01), Carter et al.
patent: 5173414 (1992-12-01), Lebkowski et al.
patent: 5354678 (1994-10-01), Lebkowski et al.
Berns et al., Advances in Virus Research, 32:243-306 (1987).
Laughlin et al., Gene, 23:65-73 (1983).
Beaton et al., J. Virol., 63(10):4450-4454 (1989).
Tratschin et al., Mol. Cell. Biol., 4(10):2072-2081 (1984).
Tratschin et al., Mol. Cell. Biol., 5(11):3251-3260 (1985).
Hermonat et al., Proc. Natl. Acad. Sci., 81:6466-6470 (1984).
McLaughlin et al., J. Virol., 62(6):1963-1973 (1988).
Samulski et al., J. Virol., 63(9):3822-3828 (1989).
Lebkowski et al., Mol. Cell. Biol., 8(10):3988-3996 (1988).
Dougherty et al., J. Virol., 63(7):3209-3212 (1989).
Markowitz et al., J. Virol., 62(4):1120-1124 (1988).
Clemens et al., J. Virol., 63(6):2737-2745 (1989).
Mendelson et al., Virol., 166:154-165 (1988).
Stary et al., J. Virol., 63(9):3837-3843 (1989).
Tratschin et al., Mol. Cell. Virol., 6(8):2884-2894 (1986).
Yates et al., Nature, 313:812-815 (1985).
Jalanko et al., Biochem. Biophys. Acta, 949:206-212 (1988).
Young et al., Gene, 62(2):171-185 (1988).
Mendelson et al., Virol., 6(2):612-615 (1988).
Jalanko et al., Gene, 78:287-296 (1989).
Konan et al., Somatic Cell and Mol. Genetics, 17(4):359-368 (1991).
Kotin et al., Proc. Natl. Acad. Sci., 87:2211-2215 (1990).
Labow et al., Mol. Cell. Biol., 7(4):1320-1325 (1987).
Labow et al., J. Virol., 62:1705-1712 (1988).
Massie et al., Mol. Cell. Biol., 6(8):2872-2883 (1986).
Ohi et al., Gene, 89:279-282 (1990).
Ruether et al., Mol. Cell. Biol., 6(1):123-133 (1986).
Russell et al., J. Virol., 66(5):2821-2828 (1992).
Samulski et al., J. Virol., 61(10):3096-3101 (1987).
Trempe et al., J. Virol., 62(1):68-74 (1988).
Weindler et al., J. Virol., 65:2476-2483 (May 1991).
Laughlin, C.A. et al., J. Virol., 41:868-876 (1982).
Muzyczka, N., Curr. Top. Microbio.. Immunol., 158:97-129 (1992).
Berkner, K.L., Biotechniques, 6:616-624 (1988).

Affiliated with

Also associated with

Rating

Recombinant adeno-associated virus vectors does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Recombinant adeno-associated virus vectors, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Recombinant adeno-associated virus vectors will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1880835