Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1995-06-06
1996-12-31
Mosher, Mary E.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
4353201, 4352351, 935 32, C12N 510, C12N 701, C12N 1586
Patent
active
055893775
ABSTRACT:
Simplified methods to produce recombinant adeno-associated virus (rAAV) vectors are described. The AAV rep and cap genes are combined in a single recombinant adenovirus vector, i.e., a rep/cap adenovirus vector which combines in a single vector all complementing functions required for rAAV production. The rep/cap vectors of the present invention comprise: genes encoding helper functions, preferably from adenovirus, sufficient to permit AAV replication; and AAV cap and rep genes. In adenovirus rep/cap vectors the rep and cap genes may, for example, replace the adenovirus E3 gene or the adenovirus E1a and E1b genes.
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Lebkowski Jane S.
McNally Maureen A.
Okarma Thomas B.
Mosher Mary E.
Rhone-Poulenc Rorer Pharmaceuticals Inc.
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