Recombinant adeno-associated viral vectors

Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Carbohydrate doai

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424 932, 424 9321, 4351723, 4353201, 435325, 435375, 935 22, 935 34, 935 62, A01N 6300, A61K 4800, C12N 500, C12N 1500

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057804478

ABSTRACT:
The present invention provides a vector comprising an enhancer element and a nuclear matrix association region inserted between an inverted terminal repeat of adeno-associated virus. The vector can further comprise a heterologous nucleic acid inserted into the vector. The present invention further provides a method for integration of a nucleic acid into the genome of a cell, comprising administering to the cell a vector comprising an enhancer element, a heterologous nucleic acid and a nuclear matrix association region, each inserted between an inverted terminal repeat of adeno-associated virus, thereby integrating the nucleic acid into the genome of the cell.

REFERENCES:
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Cunningham et al., "The Regulatory Element 3' to the .sup.A .gamma.-Globin Gene Binds to the Nuclear Matrix and Interacts With Special A-T-Rich Binding Protein 1 (SATB1), an SAR/MAR-Associating Region DNA Binding Protein", Blood 84: 1298-1308 (1994).
Bodine and Ley, "An enhancer element lies 3' to the human A.gamma. globin gene", EMBO J. 6: 2997-3004 (1987).
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Goodman et al., "Recombinant Adeno-Associated Virus-Mediated Gene Transfer Into Hematopoietic Progenitor Cells", Blood 84: 1492-1500.

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