Recipient block and method for preparation thereof

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving fixed or stabilized – nonliving microorganism,...

Reexamination Certificate

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C435S040520

Reexamination Certificate

active

07070950

ABSTRACT:
Disclosed is a recipient block for arraying a certain tissue sample into a desired position on a tissue microarray slide, comprising(a) an additive and (b) a wax. The present invention also discloses a method of preparing a recipient block, including (1) preparing an aqueous solution of an additive; (2) pouring the aqueous solution of the additive into a mold and cooling the mold to gelate the aqueous solution of the additive; (3) dehydrating the resulting additive gel in alcohol; (4) immersing the dehydrated additive gel in an organic solvent to make the dehydrated additive gel transparent; (5) penetrating a wax into the transparent additive gel to provide a block; and (6) providing a plurality of cylindrical holes in the block. Further, the present invention discloses a method of preparing a tissue microarray block, including (1) arraying each of certain tissue samples into each of the cylindrical holes of the recipient block; and (2) heating the recipient block in which the tissue samples have been arrayed at 50° C. to 70° C. for 20 to 40 min and cooling the recipient block for embedding of the tissue samples.

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patent: WO 01/51910 (2001-07-01), None
Packeisen J et al, “Demystified . . . Tissue microarray technology”-review. J. Clin. Pathol: Mol. Pathol. 2003, 56: 198-204. Entire document.
The term “additive” Merriam-Webster Online Dictionary. see at the web http:// www.m-w.com. p. 1.
Moskaluk CA and Stoler MH. “Agarose mold embedding of cultured cells for tissue microarrays”. Diagnostic Molecular Pathology, Dec. 2002 11(4): 234-238. entire document.
Packeisen J et al. “Demystified . . . Tissue microarray technology”. J. Clin. Pathol., 2003, 56: 198-204. entire document.

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