Chemistry: analytical and immunological testing – Biospecific ligand binding assay
Patent
1995-05-23
1998-06-16
Housel, James C.
Chemistry: analytical and immunological testing
Biospecific ligand binding assay
435 71, 4353171, 435817, 436512, 436518, 436524, 436525, 436532, 436547, G01N 3353, G01N 33549, G01N 33563
Patent
active
057669600
ABSTRACT:
A membrane comprising a closely packed array of self-assembling amphiphilic molecules, and is characterized in that it incorporates a plurality of ion channels, and/or at least a proportion of the self-assembling molecules comprise a receptor molecule conjugated with a supporting entity. The ion channel is selected from the group consisting of peptides capable of forming helices and aggregates thereof, corronands, cryptands, podands and combinations thereof. In the amphiphilic molecules comprising a receptor molecule conjugated with a supporting entity, the receptor molecule has a receptor site and is selected from the group consisting of immunoglobulins, antibodies, antibody fragments, dyes enzymes and lectins. "The supporting entity is selected from the group consisting of a lipid head group, a hydrocarbon chain(s), a cross-linkable molecule and a membrane protein. The supporting entity is attached to the receptor molecule at tan end remote from the receptor site. In preferred embodiments the ion channel is gramicidin A, and is preferable gated. Such membranes may be used in the formation of sensing devices.
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Huang, et al., Interactions of Immunoliposomes with Target Cells, The Journal of Biological Chemitry, No. 22, Issue of Nov. 22, pp. 14034-1440, 1983.
Braach-Maksvytis Vijoleta Lucija Bronislava
Cornell Bruce Andrew
Australian Membrane and Biotechnology Research Institute
Housel James C.
Swartz Rodney P.
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