Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1995-05-23
1997-12-02
Housel, James C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
4353171, 435817, 436501, 436512, 436518, 436524, 436525, 436532, 436547, G01N 3353, G01N 33543
Patent
active
056934777
ABSTRACT:
The present invention provides a method of detecting an analyte in a sample the method comprising adding a sample suspected of containing the analyte to a membrane comprising a closely packed array of self-assembling amphiphilic molecules, the membrane including a plurality of ion channels selected from the group consisting of peptides capable of forming helices and aggregates thereof, podands cryptands and coronands, a first receptor moiety reactive with the analyte being attached to the ion channel at an end thereof, the first receptor moiety being such that it normally exists in a first state, but when bound to the analyte exists in a second state, said change of state of the first receptor moiety causing a change in the ability of ions to pass through the ion channel, the first receptor moiety being selected from the group consisting of antigens, peptides, antibodies (e.g., immunoglobulins), antibody binding fragments, enzymes, lectins and dyes, and detecting the presence or absence of the analyte by measuring said change in the ability of ions to pass through the ion channels.
REFERENCES:
patent: 4517303 (1985-05-01), Freytag et al.
patent: 4661235 (1987-04-01), Krull et al.
patent: 4758342 (1988-07-01), Heckmann et al.
Williams, D.E., "Electrochemical Noise" and Chemical Sensing, Materials Development Division Harwell Laboratory, (One day conference in Boston, Mass., Nov. 9, 1987), pp. 203-208.
Huang, et al., Interactions of Immunoliposomes with Target Cells, The Journal of Biological Chemistry, No. 22, Issue of Nov. 22, pp. 14034-1440, 1983.
Braach-Maksvytis Vijoleta Lucija Bronislava
Cornell Bruce Andrew
Australian Membrane and Biotechnology Research Institute
Housel James C.
Swartz Rodney P.
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