Receptor function assay for G-protein coupled receptors and...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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C435S007200, C435S007210, C435S069700, C536S023400

Reexamination Certificate

active

06893827

ABSTRACT:
Methods for detecting G-protein coupled receptor (GPCR) activity; methods of assaying GPCR activity; and methods of screening for GPCR ligands, G-protein-coupled receptor kinase (GRK) activity, and compounds that interact with components of the GPCR regulatory process are described.

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patent: 6342345 (2002-01-01), Blau et al.
patent: WO 0035274 (2000-06-01), None
F. Rossi et al., “Monitoring Protein-Protein Interactions in Intact Eukaryotic Cells by β-galactosidase Complementation”, Proc. Natl. Acad. Sci., vol. 94, pp. 8405-8410, (1997).
Barak et al., “A beta-Arrestin/Green Fluorescent Protein Biosensor for Detecting G Protein-coupled Receptor Activation”, the Journal of biological Chemistry, vol. 272, No. 44, pp. 27497-27500, Oct. 31, 1997.
Stephanie Angers, et al., “Detection of β2-adrenergic receptor dimerization in living cells using bioluminescence resonance energy transfer (BRET)”, Proc. Natl. Acad. Sci. USA, vol. 97, Issue 7, pp. 3684-3689, Mar. 28, 2000.
Vsevolod V. Gurevich, et al., “Arrestin Interactions with G Protein-coupled Receptors. Direct Binding Studies of Wild Type and Mutant Arrestins with Rhodopsin, β2-Adrenergic, and m2 Muscarinic Cholinergic Receptors”, J. Biol. Chem., vol. 270, No. 2, pp. 720-731, Jan. 13, 1995.
Vsevolod V. Gurevich, et al., “Mechanism of Phosphorylation-Recognition by Visual Arrestin and the Transition of Arrestin into a High Affinity Binding State”, Mol. Pharmacol., vol. 51, pp. 161-169, 1997.

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