Receptor for a Bacillus thuringiensis toxin

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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536 235, 530350, 435325, 4352523, 43525411, C12N 1512, C12N 510, C07K 14435

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056934912

ABSTRACT:
The cDNA that encodes a glycoprotein receptor from the tobacco hornworm which binds a Bacillus thuringiensis toxin has been obtained and sequenced. The availability of this cDNA permits the retrieval of DNAs encoding homologous receptors in other insects and organisms as well as the design of assays for the cytotoxicity and binding affinity of potential pesticides and the development of methods to manipulate natural and/or introduced homologous receptors and, thus, to destroy target cells, tissues and/or organisms.

REFERENCES:
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T Ishihara et al. (1991) Molecular cloning and expression of a cDNA encoding the secretin receptor EMBO J. 10(7): 1635-1641 1991.
Gill et al., The Mode of Action of Bacillus Thuringiensis Endotoxins; Ammu. Rev. Entomol. (1992) 37:615-36.
Hoffman et al., Specificity of Bacillus Thuringiensis .delta.-endotoxins is Correlated with the Presence of High-affinity Binding Sites in the Brush Border Membrane of Target Insect Midguts; Proc. Natl. Acad. Sci. USA (1988) 85:7844-7848.
Hofte, et al., Insecticidal Crystal Proteins of Bacillus Thuringiensis; Microbiological Reviews (1989) 53(2):242-255.
Vadlamudi, et al., A Specific Binding Protein from Manduca Sexta for the Insecticidal Toxin of Bacillus Thuringiensis Subsp. Berliner; J. Biol. Chem. (1993) 268(17):12334-12340.
Van Rie et al., Specificity of Bacillus Thuringiensis .delta.-endotoxins; Eur. J. Biochem. (1989) 186:239-247.
Van Rie et al., Receptors on the Brush Border Membrane of the Insect Midgut as Determinants of the Specificity of Bacillus Thuringiensis Belta-Endotoxins (1990) 56(5):1378-1385.

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