Receptor-based histamine assay

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 21, 435241, 435188, 435810, 435 28, 436503, 436518, 436519, 436536, 436808, 436811, G01N 3354, C12Q 142, C12Q 128, C12Q 502, C12Q 996

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045446296

ABSTRACT:
A receptor-based clinical or research competitive inhibition assay method for determining histamine in body or laboratory fluids is disclosed. A kit for carrying out the method is also described comprising an amount of histamine receptors sufficient to provide an excess of receptors for binding with the amount of histamine in the sample to be assayed, and an amount of a histamine-indicator conjugate sufficient to react with the resulting unbound histamine receptors present after reaction of the histamine sample with the histamine receptors.

REFERENCES:
patent: 4299813 (1981-11-01), Snyder
patent: 4327073 (1982-04-01), Huang
Smythies et al., Receptors in Pharmacology, Marcel Dekker, Inc., N.Y., pp. 2, 7 and 123-150 (1978).
Simon, Receptors as Supramolecular Entities, Proc. Biannual Capo Boi Conf., Cagliari, Italy, Jun. 7-10, 1981, Pergamon Press, N.Y., 301, 314, 315 (1983).
Stoner, "Enzyme Affinity Assay-A New Method for the Quantitation of Histamine", Ph.D. Dissertation, University Microfilms International, Ann Arbor, Mich. (1981).
Stoner, Dissertation Abstracts International, 42(8), 3277b (Feb. 1982).
Wisdom, Clin. Chem., 22(8), 1243-1255 (1976).
Rocklin et al., Chemical Abstracts, 88: 168394x, (1978).

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