Receptor-activated reporter systems

Details Receptor-activated reporter systems Receptor-activated reporter systems

1994-08-08

1999-11-23

Sisson, Bradley

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435 691, 4353201, 435 697, 536 231, 536 235, 536 234, C12N 121

Patent

active

059898933

ABSTRACT:
The invention discloses a chimeric GLUT transporter including a GLUT receptor polypeptide fused to a domain of a detectable heterologous polypeptide and cells expressing such reporter constructs. Cells expressing a detectably-tagged chimeric GLUT transporter are used in methods of screening candidate compounds for their ability to agonize or antagonize an interaction between a ligand and a receptor, e.g., insulin. In addition, a 30 amino acid intracellular retention sequence is disclosed.

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