Chemistry: electrical and wave energy – Apparatus – Electrophoretic or electro-osmotic apparatus
Patent
1994-02-07
1996-08-06
Niebling, John
Chemistry: electrical and wave energy
Apparatus
Electrophoretic or electro-osmotic apparatus
204616, 204621, C02F 140, C02F 1100, C25B 900, C25B 1100
Patent
active
055430260
ABSTRACT:
This invention relates to an improved real-time scanning fluorescence electrophoresis apparatus for the electrophoretic analysis of fluorescently-labeled polynucleotide fragments. The apparatus is characterized by having an electrophoresis chamber containing an electrophoretic separation medium capable of accommodating multiple electrophoresis lanes arranged in a planar array, a fluorescence detector mounted on a translatable stage, a light source for exciting fluorescent molecules, and a computer for collecting data consisting of time, location, fluorescence wavelength and fluorescent intensity information. The improvements herein disclosed include, (i) using a spectral-array detector for detecting the emission light from the fluorescently-labeled polynucleotide fragments including the simultaneous detection of multiple fluorescent labels, and, (ii) a temperature control means to control the temperature of the electrophoretic separation medium during electrophoresis.
REFERENCES:
patent: 4683202 (1985-10-01), Mullis
patent: 4811218 (1986-06-01), Hunkapiller
patent: 4957613 (1990-09-01), Schuette
patent: 4975170 (1990-12-01), Hellman
patent: 5162654 (1991-02-01), Kostichka et al.
patent: 5192412 (1993-03-01), Kambara et al.
Trainor Anal.Chem., 62: 418-426 (1990). DNA Sequencing, Automation and the Human Genome. Mar. 1, 1990.
Watkins BioTechniques, 6: 310-319 (1988). no month. Restricton Fragment Length Polymorphism (RFLP): Applications in Human Chromosome Mapping and Genetic Disease Research.
Ziegle et al. Genomics, 14: 1026-1031 (1992). no month. Application of Automated DNA Sizing Technology for Genotyping Microsatellite Loci.
Brumley et al. Nucleic Acids Research, 19: 4121-4126 (1991). no month. Rapid DNA Sequencing by Horizontal Ultrathin Gel Electrophoresis.
Stegemann et al. Methods in Molecular and Cellular Biology, 2: 182-184 (1991). no month. Automated DNA Sequencing on Ultrathin Slab Gels.
Karger et al. Nucleic Acids Research, 19: 4955-4962 (1991). no month. Multiwavelength Fluorescence Detection for DNA Sequencing Using Capillary Electrophoresis.
Hoff Louis B.
Lachenmeier Eric W.
Nordman Eric S.
Raysberg Yefim M.
Grossman Paul D.
Niebling John
The Perkin-Elmer Corporation
Wong Edna
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