Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Reexamination Certificate
2003-11-28
2009-02-17
Horlick, Kenneth R. (Department: 1637)
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
C435S091200
Reexamination Certificate
active
07491814
ABSTRACT:
The present invention relates to a highly sensitive real-time RT-PCR method for specifically detecting the expression of more than one MAGE gene. The present invention further relates to a diagnostic composition for carrying out such a real-time RT-PCR as well as to oligonucleotides suitable for the cDNA synthesis reaction prior to real-time PCR amplification of more than one marker from the MAGE gene family. To enable the quantitative measurement of MAGE gene expression in a clinical sample an RT-protocol was invented using very sophisticated non-standard conditions to accomplish real-time PCR amplification of cDNA of several MAGE family members in relation to a comparative normalizing reference gene as internal control.
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Kufer Peter
Mecklenburg Ingo
Baughman Molly E
Fulbright & Jaworski LLP
Horlick Kenneth R.
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