Real-time polymerase chain reaction-based genotyping assay...

Details Real-time polymerase chain reaction-based genotyping assay... Real-time polymerase chain reaction-based genotyping assay...

2006-03-28

2006-03-28

Switzer, Juliet C. (Department: 1634)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S006120, C536S024310, C536S024300, C536S023100

Reexamination Certificate

active

07018816

ABSTRACT:
The present invention provides fluorescence-based real-time PCR assays for the rapid detection of single nucleotide polymorphisms (SNPs). The genotyping assay can be used to detect SNPs of a number of genes of interest that include, but are not limited to, the human multidrug resistance gene (MDR1) single nucleotide polymorphisms C3435T and G2677T, and cytochrome P-450 3A5 single nucleotide polymorphisms CYP3A5*3 (A22893G) and CYP3A5*6 (G30597A).

REFERENCES:
Song et al. (AAPS PharmSci 2002; 4(4) article 29 (http://www.aapspharmsci.org), published Oct. 2, 2002).
Song et al. (Clinical Pharmacology & Therapeutics, vol. 71, No. 2, Feb. 2002, p. P103, abstract WPIII-100).
Hoffmeyer et al. (PNAS, vol. 97, No. 7, pp. 3473-3478, Mar. 28, 2000).
GenBank M14758 (GI: 187468, Dec. 3, 1999).
Okimoto et al. (Bio Techniques, vol. 21, Jul. 1996), pp. 20,22, 24, 26.
GenBank AC005068 (GI: 10122135, Oct. 7, 2000).

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