Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2007-08-28
2007-08-28
Fredman, Jeffrey (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
Reexamination Certificate
active
10283346
ABSTRACT:
Ralstonia solanacearum, the causal agent of bacterial brown rot of potato, is often carried latently in seed potato tubers. Primers and a probe were designed for a real-time BIO-PCR assay technique for detecting potato tubers latently infected withR. Solanacearum. Using naturally infected potato tubers, as few as 20 cells/ml extract could be detected. Two of 14 naturally infected potato tubers with no disease symptoms were positive by the newly described real-time BIO-PCR (pre-enrichment on agar or in liquid medium) assay but not by direct real-time PCR.
REFERENCES:
Stratagene Catalog, 1988.
Boehringer Mannheim Biochemicals Catalog, 1997.
Ito et al. Fluorescent differential display: arbitrarily primed RT-PCR fingerprinting on an automated DNA sequencer. 1994. FEBS Letters vol. 351:231-236.
Roest-Crollius et al. Genebank Accession No. AL234392.
Genebank accession Nos. AE005991.1, AP008207.1 and AP003045.2 pp. 1-3.
Genebank accession Nos. AE016814.1, AE005827.1, AF210625.2 and NM—207880.1 pp. 1-3.
Ciampi, L., et al., “Latent Infection of Potato Tubers byPseudomona solanacearum”, Am. Potato J., vol. 57, pp. 377-386, Jan. 22, 1980.
Fegan, M., et al., “Phylogeny, Diversity and Molecular Diagnostics ofRalstonia solanacearum”, In: Bacterial Wilt Disease: Molecular and Ecological Aspects, pp. 19-33, 1998.
Fegan, M., et al., “Development of a Diagnostic Test Based on the Polymerase Chain Reaction (PCR) to Identify Strains ofR. solanacearumExhibiting the Biovar 2 Genotype”,In: Bacterial Wilt Disease: Molecular and Ecological Aspects, pp. 34-43, 1998.
Frederick, R., et al., “Identification and Differentiation ofTilletia indicaandT. WalkeriUsing the Polymerase Chain Reaction”,Phytopathology, vol. 90, pp. 951-960, May 22, 2000.
Hayward, A.C., et al., “Round Table on Bacterial Wilt (Brow Rot) of Potato”,In: Bacterial Wilt Disease: Molecular and Ecological Aspects, pp. 420-429, 1998.
Schaad, N.W., et al., “Real-Time Polymerase Chain Reaction for One-Hour On-Site Diagnosis of Pierce's Disease of Grape in Early Season Asymptomatic Vines”,Phytopathology, vol. 92, (7), pp. 721-728, 2002.
Schoen, C.D., et al., “Detection of Potato Leafroll Virus in Dormant Potato Tubers by Immunocapture and a Fluorogenic 5′ Nuclease RT-PCR Assay”,Phytopathology, vol. 86, (9), 1996.
Weller, S.A., et al., “Detection ofRalstonia solanacearumStrains with a Quantitative Multiplex, Real-Time, Fluorogenic PCR (TaqMan) Assay”,Applied and Environmental Microbiology, vol. 66, (7), pp. 2853-2858, Jul. 2000.
Zhang, A.W., et al., “Molecular Detection ofDiaporthe phaseolorumandPhomopsis longicollafrom Soybean Seeds”,Phytopathology, vol. 89, (9), pp. 796-804, 1999.
Gaush Phillip E.
Ozakman Meric
Schaad Norman W.
Calamita Heather G.
Fado John D.
Fredman Jeffrey
Rabin Evelyn M.
The United States of America as represented by the Secretary of
LandOfFree
Real-time PCR primers and probes for identification of... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Real-time PCR primers and probes for identification of..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Real-time PCR primers and probes for identification of... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3894478