Real-time PCR assay of the bacterium Edwardsiella ictaluri...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C435S091200, C536S023100, C536S024300, C536S024320, C536S024330

Reexamination Certificate

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06951726

ABSTRACT:
A rapid and sensitive PCR-based assay is provided to facilitate early detection ofEdwardsiella ictaluriin channel catfish. This bacteria is the causative agent in the disease, Enteric septicemia of catfish (ESC). Also provided is a method of selecting breeding stock for use in selective breeding programs to improve disease resistance in channel catfish. Also provided is a method of determining the efficacy of vaccines produced against ESC.

REFERENCES:
patent: 6316195 (2001-11-01), Frederick et al.
patent: 6355435 (2002-03-01), Wilson et al.
patent: 6387652 (2002-05-01), Haugland et al.
Baldwin, T.J., et al., “Pathogenesis of Enteric Septicemia of Channel Catfish, Caused byEdwardsiella ictaluri: Bacteriologic and Light and Electron Microscopic Findings”, Journal of Aquatic Animal Health, 5, 189-198 (1993).
Bell, K.S., et al., “Detection and Quantification ofSpongospora subterraneaf. sp.Subterraneain Soils and on Tubers Using Specific PCR Primers”, European Journal of Plant Pathology, 105, 905-915 (1999).
Francis-Floyd, R., et al., “Effect of Water Temperature on the Clinical Outcome of Infection withEdwardsiella ictaluriChannel Catfish”, JAVMA, 191, 11, 1413-1416 (1987).
Hawke, J.P., et al., “Edwardsiella ictalurisp. nov., the Causative Agent of Enteric Septicemia of Catfish”, Int'l. Journal of Systematic Bacteriology, 31, 4, 396-400 (1981).
Kimura, B., et al., “Rapid, Quantitative PCR Monitoring of Growth ofClostridium botulinumType E in Modified-Atmosphere-Packaged Fish”, Applied and Environmental Microbiology, 67, 1, 206-216 (2001).
Klesius, P., “Transmission ofEdwardsiella ictalurifrom Infected, Dead to Noninfected Channel Catfish”, Journal of Aquatic Animal Health, 6, 180-182 (1994).
Klesius, P., et al., “Development and Evaluation of an Enzyme-Linked Immunosorbent Assay for Catfish Serum Antibody toEdwardsiella ictaluri”, Journal of Aquatic Animal Health, 3, 94-99 (1991).
Leal-Klevezas, D.S., et al., “Single-Step PCR for Detection ofBrucellaspp. from Blood and Milk of Infected Animals”, Journal of Clinical Microbiology, 33, 12, 3087-3090 (1995).
León, G., et al., “A PCR-Based Assay for the Identification of the Fish PathogenRenibacterium salmoninarum”, FEMS Microbiology Letters, 115, 131-136 (1994).
Makino, S-I, et al., “Direct Detection ofBacillus anthracisDNA in Animals by Polymerase Chain Reaction”, Journal of Clinical Microbiology, 31, 3, 547-551 (1993).
Miriam, A., et al., “PCR and Probe-PCR Assays To Monitor Broodstock Atlantic Salmon (Salmo solarL.) Ovarian Fluid and Kidney Tissue for Presence of DNA of the Fish PathogenRenibacterium salmoninarum”, Journal of Clinical Microbiology, 35, 6, 1322-1326 (1997).
Newton, J.C., et al., “Pathology of Experimental Enteric Septicaemia in Channel Catfish,Ictalurus punctatus(Rafinesque), Following Immersion-Exposure toEdwardsiella ictaluri”, Journal of Fish Diseases, 12, 335-347 (1989).
Nogva, H.K., et al., “Application of the 5′-Nuclease PCR Assay in Evaluation and Development of Methods for Quantitative Detection ofCampylobacter jejuni”, Applied and Environmental Microbiology, 66, 9, 4029-4036 (2000).
Nogva, H.K., et al., “Detection and Quantification ofSalmonellain Pure Cultures Using 5′-Nuclease Polymerase Chain Reaction”, International Journal of Food Microbiology, 51, 191-196 (1999).
Plumb, J.A., et al., “Survival ofEdwardsiella ictaluriin Pond Water and Bottom Mud”, The Progressive Fish-Culturist, 48, 212-214 (1986).
Rogers, W.A., “Serological Detection of Two Species ofEdwardsiellaInfecting Catfish”, Int'l Symposium on Fish Biologies: Serodiagnostics and Vaccines, 49, 169-172 (1981).
Shoemaker, C.A., et al., “Protective Immunity Against Enteric Septicaemia in Channel Catfish,Ictalurus punctatus(Rafinesque), Following Controlled Exposure toEdwardsiella ictaluri”, Journal of Fish Diseases, 20, 361-368 (1997).
Shotts, E.B., et al., “Pathogenesis of ExperimentalEdwardsiella ictaluriInfections in Channel Catfish (Ictalurus punctatus)”, Can. J. Fish Aquat. Sci., 43, 36-42 (1986).
Vishnubhatla, A., et al., “Rapid 5′ Nuclease (TaqMan) Assay for Detection of Virulent Strains ofYersinia enterocolitica”, Applied and Environmental Microbiology, 66, 9, 4131-4135 (2000).
Wise, D.J., et al., “Effect of Feeding Frequency and Romet-Medicated Feed on Survival, Antibody Response, and Weight Gain of Fingerling Channel CatfishIctalurus punctatusAfter Natural Exposure toEdwardsiella ictaluri”, Journal of the World Aquaculture Society, 29, 2, (1998).

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