Real-time impedance assay to follow the invasive activities...

Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of...

Reexamination Certificate

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Reexamination Certificate

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10938086

ABSTRACT:
The present invention provides methods for the assessment of the metastatic potential of cells by measuring the effect of the cells on the impedance of an electrode. Accordingly, the present invention similarly provides methods for establishing indicators of metastatic potential based on a cell's effect on impedance. Such methods may be employed in vitro or in vivo. In some embodiments, the electrode is coated with endothelial or epithelial cells.

REFERENCES:
patent: 5187096 (1993-02-01), Giaever et al.
Morimoto et al. J of Investigative Surgery1993;6:25-32.
Zak et al. Pflugers Arch-Eur J Physiol 2000;440:179-183.
Smith et al. IEEE Transactions on Biomedical Engineering 2000;47:1403-1405.
Zak et al. Eur J Physiol 2000;440:179-183.
Depaola, N. et al., “Electrical Impedance of Cultured Endothelium Under Fluid Flow,” Annals of Biomedical Engineering, vol. 29, pp. 1-9, 2001.
Wegener, J. et al., “Electric Cell-Substrate Impedance Sensing (ECIS) as a Noninvasive Means to Monitor the Kinetics of Cell Spreading to Artificial Surfaces,” Experimental Cell Research 259, pp. 158-166, 2000.
Giaever, I. et al., “A Morphological Biosensor for Mammalian Cells,” Nature, vol. 366, pp. 591-592, Dec. 9, 1993.
Giaever, I. et al., “Monitoring Fibroblast Behavior in Tissue Culture With an Applied Electric Field,” Proc. Natl. Acad. Sci. USA, vol. 81, pp. 3761-3764, Jun. 1984.
Keese, CR et al., “A Biosensor that Monitors Cell Morphology with Electrical Fields,” IEEE Engineering in Medicine and Biology, pp. 402-408, Jun./Jul. 1994.
Reddy, L. et al., “Assessment of Rapid Morphological Changes Associated with Elevated cAMP Levels in Human Orbital Fibroblasts,” Experimental Cell Research 245, pp. 360-367, 1998.
Keese, CR. et al., “Cell-Substratum Interactions as a Predictor of Cytotoxicity,” In Vitro & Molecular Toxicology, vol. 11, No. 2, pp. 183-192, 1998.
Giaever, I. et al., “Micromotion of Mammalian Cells Measured Electrically,” Proc. Natl. Acad. Sci. USA, vol. 88, pp. 7896-7900, Sep. 1991.
Mitra, P. et al., “Electric Measurements Can be Used to Monitor the Attachment and Spreading of Cells in Tissue Culture,” BioTechniques (The Journal of Laboratory Technology for Bio-research), vol. 11, No. 4, pp. 504-510, Oct. 1991.
Tiruppathi, C. et al., “Electrical Method for Detection of Endothelial Cell Shape Change in Real Time: Assessment of Endothelial Barrier Function,” Proc. Natl. Acad. Sci. USA, vol. 89, pp. 7919-7923, Sep. 1992.
Keese, CR et al., “Real-Time Impedance Assay to Follow the Invasive Activities of Metastatic Cells in Culture,” BioTechniques, vol. 33, No. 4, pp. 842-850, 2002.

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