Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1996-07-26
1998-12-29
Horlick, Kenneth R.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 6, 435 912, 536 243, 536 2532, C12Q 168, C12P 1934, C07H 2104, C07H 2100
Patent
active
058539905
ABSTRACT:
A method for performing real time, homogeneous assay of a target nucleic acid comprising annealing a labeled, ribo-oligonucleotide probe to the target DNA sequence and degrading a portion of the probe with RNase H to release labeled fragments. Sequential measurements of the released fragments permits kinetic characterization of the presence of the target sequence. Preferably, the assay is integrated into a polymerase chain reaction so that target amplification can be detected in real time.
REFERENCES:
patent: 3996345 (1976-12-01), Ullman et al.
patent: 3998943 (1976-12-01), Ullman
patent: 4160016 (1979-07-01), Ullman
patent: 4174384 (1979-11-01), Ullman et al.
patent: 4199559 (1980-04-01), Ullman et al.
patent: 4683194 (1987-07-01), Saiki et al.
patent: 4683202 (1987-07-01), Mullis
patent: 4800159 (1989-01-01), Mullis et al.
patent: 4876187 (1989-10-01), Duck et al.
patent: 4889818 (1989-12-01), Gelfand et al.
patent: 4914210 (1990-04-01), Levenson et al.
patent: 5011769 (1991-04-01), Duck et al.
patent: 5210015 (1993-05-01), Gelfand et al.
patent: 5244797 (1993-09-01), Kotewicz et al.
patent: 5487972 (1996-01-01), Gelfand et al.
Mullis and Faloona, Methods in Enzymol., vol. 155, p. 335 (1987).
Livak et al., PCR Methods and Applications, 4:357 (1995).
Duck et al., Bio Techniques, 9;142 (1990).
Bekkaoui et al., Bio Techniques, 20:240 (1996).
Hargrove David E.
Kessler Donald J.
Winger Edward E.
Horlick Kenneth R.
Koenig Nathan P.
Tung Joyce
Winger Edward E.
LandOfFree
Real time homogeneous nucleotide assay does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Real time homogeneous nucleotide assay, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Real time homogeneous nucleotide assay will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1423321