Real-time gene quantification with internal standards

Details Real-time gene quantification with internal standards Real-time gene quantification with internal standards

2002-08-29

2009-12-08

Zhou, Shubo (Joe) (Department: 1631)

Data processing: measuring, calibrating, or testing

Measurement system in a specific environment

Biological or biochemical

C702S020000, C702S027000, C435S006120, C435S091100, C435S091200, C536S023100, C536S024300, C536S024330

Reexamination Certificate

active

07630837

ABSTRACT:
The present invention is directed to a nucleic acid quantification kit and method for determining the initial concentration or mass fraction of a target nucleic acid present in a sample. Illustrative embodiments include real-time competitive quantitative polymerase chain reaction (PCR) to determine the copy number or mass fraction of a target nucleic acid sequence in a sample and use of a thermodynamically based signal processing algorithm, with or without PCR, to provide mass fraction information.

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