Real time detection of intermolecular interaction

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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C435S007920, C436S501000, C436S518000, C530S387100

Reexamination Certificate

active

06936427

ABSTRACT:
Intermolecular binding can be detected by formation of a “paratope” which results in an immediate generation of a signal. The substances to be tested for interaction are bound to demitopes, wherein said demitopes are components of a paratope which binds a reporter which provides said signal when bound. Known interactions measured in this way can also be employed to screen for compounds which interfere with the interactions. In addition to testing for individual interactions, the interaction of a compound with a library or library×library interactions can also be determined and the effect of potentially interfering substances evaluated.

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Arndt et al., helix-stabilized Fv (hsFv) antibody Fragments: Substituting the Constant domains of a Fab Fragment for a heterodimeric coiled-coil Domain, J. Mol. Biol. (2001) 312, 221-228.
Kranz et al., restricted reassociation of heavy and light chains from hapten-specific monoclonal antibodies, Proc. Natl. Acad. sci. USA, vol. 78, No. 9, pp. 5807-5811, 1981.
Griffin et al., Specific Covalent Ibeling of Recombinant Protein Molecules Inside Live cells, Science, vol. 281, Jul. 10, 1998, pp. 269-271.

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