Real-time, 3D, non-linear microscope measuring system and...

Optics: measuring and testing – By dispersed light spectroscopy – With sample excitation

Reexamination Certificate

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C356S417000, C250S458100

Reexamination Certificate

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07872748

ABSTRACT:
A real-time, 3D, non-linear microscope measuring system and method for examining a set of microscopic image points in different image planes. The system comprises a pulsed laser or parametric oscillator light source generating an examining optical signal, and is applicable to measure and/or photochemically stimulate pre-selected points within a short time interval. The system further comprises a bundle of fibers composed of optical fibers or other waveguides, a rapidly working optical switch, a imaging system, a light source and an optical system. The examining optical signal is a fluorescent or other optical signal imaged on the required spot.

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Denk et al., “Two-photon molecular excitation in laser-scanning microscopy,” Handbook of Biological Confocal Microscopy, 1995, pp. 445-448, XP001040853, ISBN: 0-306-44826-2.

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