Chemistry: analytical and immunological testing – Involving immune complex formed in liquid phase – Signal modification or steric inhibition
Patent
1993-06-29
1994-10-11
Cererley, Mary E.
Chemistry: analytical and immunological testing
Involving immune complex formed in liquid phase
Signal modification or steric inhibition
436546, 436816, G01N 33542
Patent
active
053546938
ABSTRACT:
A fluorescence polarization immunoassay (FPIA) for detecting the presence of one or more amphetamine-class analytes in a test sample is provided. The immunoassay uses competition between the analyte and a fluorescently labeled tracer for the binding site on an antibody specific for phenethylamine derivatives. The concentration of amphetamine-class analyte in the sample determines the amount of tracer that binds to the antibody. The amount of tracer-antibody complex formed can be quantitatively measured and is inversely proportional to the quantity of analyte in the test sample. The invention relates to tracers, to immunogens used to elicit antibodies for use as assay reagents, and to assay kits incorporating these tracers and assay reagents.
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Brynes Paul J.
Flentge Charles A.
Johnson Donald D.
Jonas Patrick F.
Molina Cynthia M.
Abbott Laboratories
Cererley Mary E.
Steele Gregory W.
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