Reagents for the enzymatic determination of triglycerides

Details Reagents for the enzymatic determination of triglycerides Reagents for the enzymatic determination of triglycerides

1978-07-13

1980-09-16

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 25, 435 26, 435190, C12Q 144

Patent

active

042230900

ABSTRACT:
A method of determining triglycerides in biological fluids according to an enzymatic reaction. In the reaction, a biological fluid is added to an enzyme reagent comprising a lipase, glycerol dehydrogenase (GDH), pyridine nucleotide (PN), a buffer, a tetrazolium compound and diaphorase, whereby the triglycerides are enzymatically hydrolyzed, and the resulting glycerol is oxidized to form a reduced pyridine nucleotide (PN) which reacts with said tetrazolium compound in the presence of the diaphorase to produce a colored formazan which is the reduced form of the tetrazolium, which color is measured at a wavelength ranging from about 475 to about 325 nm. The lipase is an enzyme obtained from Chromobacterium viscosum. In the enzyme reagent, there is from about 5.0 to about 15.0 I.U./ml of the glycerol dehydrogenase (GDH) present.

REFERENCES:
patent: 3703591 (1972-11-01), Bucolo et al.
patent: 4038146 (1977-07-01), Nonaka et al.
patent: 4142938 (1979-03-01), Stavropoulos et al.
Lin, et al, "The Activiation of Glycerol Dehydrogenase from Aerobacter aerogenes by MonovalentCations", J. Biol. Chem., vol. 235, No. 6, (1960), pp. 1820-1823.

Affiliated with

Also associated with

Rating

Reagents for the enzymatic determination of triglycerides does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Reagents for the enzymatic determination of triglycerides, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Reagents for the enzymatic determination of triglycerides will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-541226