Reagents for the determination of enzymes

Details Reagents for the determination of enzymes Reagents for the determination of enzymes

1982-01-22

1984-09-18

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 4, 435 22, 435 21, 435810, 435188, C12Q 134, C12Q 100, C12Q 140, C12Q 142, C12N 996

Patent

active

044724993

ABSTRACT:
Assay reagents for the determination of enzymes such as .alpha.-amylase, .alpha.-glucosidase, .beta.-glucosidase, and acid and alkaline phosphatases are described. The reagents comprise a substrate capable of releasing the chromophore p-nitrophenol at a rate proportional to the amount of enzyme being assayed and a buffer material that eliminates the temperature dependence of the chromophore.

REFERENCES:
patent: 4102747 (1978-07-01), Duiscoll
patent: 4145527 (1979-03-01), Burns et al.
patent: 4225672 (1980-09-01), Hall
patent: 4233403 (1980-11-01), Menson et al.
patent: 4243753 (1981-01-01), Regnier et al.
Wilkinson, The Principles and Practice of Diagnostic Enzymology, Year Book Medical Publishers, Inc., Chicago, 143-144, (1976).
Good et al., Biochemistry, 5, (2), 467-477, (1966).

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